Joon sik Yoon, Hei Chan Lee, Tae-Jin Oh, Kwangkyoung Liou, Jae Kyung Sohng
언어
영어(ENG)
URL
https://www.earticle.net/Article/A192434
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원문정보
초록
영어
Sialic acid containing structures in eukaryotic systems play important roles in a variety of physiological and pathological processes, including cell–cell interactions, inflammation, fertilization, viral infection, differentiation, malignancies, and cell signaling etc. Especially sialic acid derivatives product in living system are spotlighted because sialic acid is an intermediate to inhibit viral disease like SARS, bird flu infection is reported. Although, sialytransferase has been expressed in E.coli, because of its toxicity many problems arise in use to animal or human. In this study, to express sialytransferase gene from Pasteurella multocida in yeast, a non-toxic widely used strain. Cloning was carried out in yeast shuttle vector pYES2 and expressed. Enzymatic activity was analyzed by TLC and Bio-LC.
저자
Joon sik Yoon [ Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, SunMoon University ]
Hei Chan Lee [ Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, SunMoon University ]
Tae-Jin Oh [ Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, SunMoon University ]
Kwangkyoung Liou [ Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, SunMoon University ]
Jae Kyung Sohng [ Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, SunMoon University ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.