Earticle

초록

영어

The mAb CO17-1A recognizes the colorectal antigen GA733-2E, which highly is expressed on the surface membrane of human colorectal carcinoma cells. We have successfully developed plant system for production of anti-colorectal cancer monoclonal antibody, mAb CO17-1A. Plant expression system for mAb production has several advantages in terms of the lack of animal pathogenic contaminants, low cost, ease of scale-up for agricultural production, and efficient post-translational glycosylation modifications compared to the conventional fermentation methods. KDEL (Lys-Asp-Glu-Leu), ER retention signal attached to C-terminus of protein might induce high protein expression and the glycoprotein carrying oligomannose glycans. In this study, the expression level of mAb CO17-1A was compared between mAbs fused with and without KDEL in plant. PCR analysis showed stable gene insertion of heavy and light chain genes of mAb CO17-1A with or without KDEL. We validated the expression of mAb CO17-1A by western blot and its subcellular localization under the confocal microscopy. Western blot showed the significantly increased expression level of mAb CO17-1A with KDEL compared to without KDEL. Cell ELISA with human colorectal carcinoma cell line confirmed that expression of the mAb with KDEL was significantly higher than that of mAb without KDEL. The glycosylation analysis revealed that mAb CO17-1A without KDEL had plant specific glycans structure while mAb CO17-1A with KDEL had mainly oligomannose type glycans. Taken together, these studies suggest that KDEL affects expression of mAb and its glycan structures.

저자

• Yangkang So [ Department of Biological Science, Wonkwang University, Iksan, Chonbuk, 570-749, Korea ]
• Zhe Lu [ Department of Biological Science, Wonkwang University, Iksan, Chonbuk, 570-749, Korea ]
• Eun-Yi Oh [ Department of Biological Science, Wonkwang University, Iksan, Chonbuk, 570-749, Korea ]
• Da-Young Park [ Department of Biological Science, Wonkwang University, Iksan, Chonbuk, 570-749, Korea ]
• Kyung-Jin Lee [ Department of Biological Science, Wonkwang University, Iksan, Chonbuk, 570-749, Korea, Korean Research Institute of Bioscience & Biotechnology, Daejeon, 305-806, Korea ]
• Mira Song [ Department of Biological Science, Wonkwang University, Iksan, Chonbuk, 570-749, Korea ]
• Seung Han Oh [ Department of Agricultural Biology, Chonnam National University ]
• Arshad Jamal [ Department of Biological Science, Wonkwang University, Iksan, Chonbuk, 570-749, Korea ]
• Mi-Hyun Ahn [ Department of Biological Science, Wonkwang University, Iksan, Chonbuk, 570-749, Korea ]
• Yeon Soo Han [ Department of Agricultural Biology, Chonnam National University, Gwangju, 500-757, Korea ]
• Doo-Byoung Oh [ Korean Research Institute of Bioscience & Biotechnology, Daejeon, 305-806, Korea ]
• Youngkwan Kim [ Department of Biological Science, Wonkwang University, Iksan, Chonbuk, 570-749, Korea ]
• Kisung Ko [ Department of Biological Science, Wonkwang University, Institute of Biotechnology, Wonkwang University, Iksan, Chonbuk, 570-749, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614