Earticle

초록

영어

N-glycosylation is a major post-translational protein modification, which alters physicochemical properties of the protein, affecting the folding, distribution, stability and thus biological function and efficiency of protein. Plant type complex N-glycans are distinctive from those found in mammalian because they contain β1,2-xylose and core α 1,3-fucose residues attached to the pentasaccharide (Man3GlcNAc2) core structure but no sialic acid residues. The presence of β1,2-xylose and core α1,3-fucose residues on plant type complex N-glycans has long been an irritating limitation in the use of plant-made pharmaceuticals (PMPs) in human therapy, as these N-glycan epitopes are potentially immunogenic in mammals. In this study, to remove the plant specific sugar residues and humanize the N-glycosylation in plant, we isolated mutants of the corresponding plant specific glycosyltransferase genes (α1,3-fucosyltransferase I, α1,3-fucosyltransferase II, β 1,2-xylosyltransferase, β1,3-galactosyltransferase and α1,4-fucosyltransferase). We made double, triple and quadruple mutants by crossing the mutants, and quintuple mutant is on the construction. The triple (fuct1/fuct2/xylt) and quadruple (fuct1/fuct2/xylt/fuct3) mutants do not show significant developmental defects in a normal growth condition and they do not produce the plant specific sugar residues on the N-glycan. The resulting mutant will be transformed by human α1,6-fucosyltransferase and β1,4-galactosyltransferase genes to accomplish further humanized N-glycosylation in plant. [Supported by BK21 program]

저자

• Bo Hwa Son [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
• In Jung Jung [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
• Jeong Chan Moon [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
• Joo Mi Jeon [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
• Wahyu Indra Fanata [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
• Jae Yong Yoo [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
• Jae Ho Cha [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
• Je Hein Kim [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
• Rikno Harmoko [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
• Ki Seong Ko [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
• Sang Yeol Lee [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
• Kyun Oh Lee [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614