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Quantitative Analysis of Glycan Microarrays for Assaying Galactosyltransferase Activity
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- 발행기관
- 한국당과학회 바로가기
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- 간행물
- 한국당과학회 학술대회 바로가기
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- 통권
- 2008 Eastern Asian Glycoscience Symposium (2008.11)바로가기
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- 페이지
- pp.72-73
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A192360
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
- 영어
- Glycan microarrays have been used recently as an advanced technology for the high-throughput analysis of protein-carbohydrate interactions and for the detection of cells and pathogens. A major driving force behind the expanded use of this microarray technology is the development of new experimental protocols in which they can be applied. As a continuous effort to extend the use of this technology, carbohydrate microarrays were applied for assaying glycosyltransferase activities. The microarray-based approach requires only a small amount of immobilized acceptor substrates (picomoles), and enzymatic catalytic activities are facilely analyzed by measuring the amount of a product formed in a time-dependent manner. The level of time-dependent product conversion is determined by using fluorescence detection of lectin recognition of carbohydrate products. Carbohydrate microarrays immobilized twenty glycan probes were prepared by immobilizing hydrazide-conjugated carbohydrates on epoxide-coated glass slides. Treatment of glycan microarrays with β-1,4-galactosyltransferase in the presence of UDP-Gal and Mn2+ showed that α- and β-GlcNAc were converted to α- and β-LacNAc, respectively. In addition, this experiment indicated that β-GlcNAc was a better substrate than α-GlcNAc for this enzyme, which was also confirmed by HPLC analysis of enzymatic product of each substrate and solution-based assay. Furthermore, quantitative binding affinities (Kd values) between α-LacNAc or β-LacNAc and RCA120 were determined by using carbohydrate microarrays. The Kd values (34 nM for α-LacNAc and 33 nM for β-LacNAc) determined from carbohydrate microarrays were similar to those (26.3 nM for α-LacNAc and 25.3 nM for β-LacNAc) obtained by using a conventional SPR technology. These findings should open new applications of carbohydrate microarrays in the field of glycomics research.
저자
간행물 정보
발행기관
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- 발행기관명
- 한국당과학회 [Korean Society for Glycoscience]
- 설립연도
- 2006
- 분야
- 의약학>약학
- 소개
- 본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.
간행물
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- 간행물명
- 한국당과학회 학술대회
- 간기
- 연간
- 수록기간
- 2006~2022
- 십진분류
- KDC 517 DDC 614
이 권호 내 다른 논문 / 한국당과학회 학술대회 2008 Eastern Asian Glycoscience Symposium
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