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Session III: Microbial Glycosciences - Bioactive Carbohydrates & Enzymes, Chair : Dr. Do-Man Kim (Chonnam National Univ., Korea)
Alpha-1,6 Glucan Active Enzymes for Carbohydrate Engineering and Their Application
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- 발행기관
- 한국당과학회 바로가기
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- 간행물
- 한국당과학회 학술대회 바로가기
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- 통권
- 2008 Eastern Asian Glycoscience Symposium (2008.11)바로가기
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- 페이지
- pp.27-28
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A192301
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
- 영어
- Endo-dextranases (EC 3.2.1.11), which hydrolyze -1,6-glucosidic linkage of dextran at random, are classified into glycoside hydrolase (GH) family 49 and 66 based on the amino-acid sequence similarity. Cycloisomaltodextrin glucanotransferase (CITase) is also classified into GH family 66. CITase is an interesting enzyme that catalyzes the conversion of dextran to cycloisomaltodextrins (CIs) by intramolecular transglycosylation. CI is of importance by preventing the dental caries since it strongly inhibits insoluble glucan formation by mutansucrase. Although endo-dextranase and CITase belong to GH family 66, their products from dextran are largely different in structure, such as cyclic- or linear-isomaltooligosaccharides, respectively. In this study, it was found that Paenibacillus sp. dextranase, a member of GH family 66, showed both of endo-dextranase activity and CITase activity, allowing us to classify the GH family 66 enzymes to three groups for the first time. These results may contribute to understanding of the relationship between the structure and function of GH family 66 enzymes. Recently, several other Bacillus and Paenibacillus CI-producing bacterial strains were found and the larger CI molecules up to CI-17 were isolated from their culture supernatants. In order to produce CIs for commercial scale, a high dextran producing strain Leuconostoc sp. S-51 was obtained and the B. circulans T-3040 strain was mutated to produce CITase with about 110 times the activity of the wild type strain. All CIs well inhibit glucosyltransferase of mutans streptococci and especially CI-10 and larger molecules of CIs have strong inclusion-forming activity such as preservation of Victoria blue color or solubilization of isoflavones. There have been no problems arising from safety tests of CIs in rats and humans. The fact that CIs are present in brown sugar is indicative if their safety as natural oligosaccharides.
저자
간행물 정보
발행기관
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- 발행기관명
- 한국당과학회 [Korean Society for Glycoscience]
- 설립연도
- 2006
- 분야
- 의약학>약학
- 소개
- 본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.
간행물
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- 간행물명
- 한국당과학회 학술대회
- 간기
- 연간
- 수록기간
- 2006~2022
- 십진분류
- KDC 517 DDC 614
이 권호 내 다른 논문 / 한국당과학회 학술대회 2008 Eastern Asian Glycoscience Symposium
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