현재 위치
Home
Session II : Current Issues in Glycobiology and Its Application I , Chair : Dr. Jeong Heon Ko (KRIBB, Korea)
Tissue Specific Regulation of Glycolipids and Related Functions
-
- 발행기관
- 한국당과학회 바로가기
-
- 간행물
- 한국당과학회 학술대회 바로가기
-
- 통권
- 2008 Eastern Asian Glycoscience Symposium (2008.11)바로가기
-
- 페이지
- pp.14-15
-
- 저자
-
- 언어
- 영어(ENG)
-
- URL
- https://www.earticle.net/Article/A192294
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
- 영어
- The expression of glycan chains and hydrophobic structures of glycolipids is precisely regulated in a time- and space-dependent manner. We focus our research on two genes, Gsl5 (glycosphingolipid regulatory gene 5) and Des2 (degenerative spermatocyte 2). Gsl5 regulates the expression of GlcNAc 1-6(Gal 1-3)GalNAc containing glycolipids and glycoproteins through controlling the activity of 6GlcNAc transferase in mouse kidney. Des2 encodes a hydroxylase which hydroxylates dihydroceramide:sphinganine at C4 position and is responsible for the expression of glycolipids containing C4 hydroxysphinganine (phytosphingosine) in the small intestine and kidney of mice. We found that Gsl5 locates at about 5.5 kb upstream of exon 1 of 6GlcNAc T1, consists of eight pairs of two GT-repeats, and controls 6GlcNAc T1 mRNA expression in kidney proximal tubular cellspecific manner. 6GlcNAc transferase transfers GlcNAc on the GalNAc of Gal 1-3GalNAc and of glycolipids and glycoproteins, respectively. One of glycoproteins modified by the GlcNAc transferase in the proximal tubular epithelial cells is megalin, which binds and reabsorbs small molecular weight proteins from urine produced by the blood filtration at glomeruli. We purified two types of megalin, one carries Gal 1-4(Fuc 1-3)GlcNAc 1-6(Gal 1- 3)GalNAc 1-Ser/Thr (Lewis X-core 2 structure) and the other the structure without the Gal 1-4(Fuc 1-3)GlcNAc 1-6 branch from mouse kidneys. Ligand binding capacity of the megalin with Lewis Xcore 2 structure was much higher than that without the branch structure. Des2 hydroxylase requires NADH, NADH-dependent cytochrome b5 reductase, and cytochrome b5 for producing the activity. mRNA expression of Des2 is regulated in a tissue-specific manner, highest in the small intestine and high in the kidney. We are in the process of establishing Des2 knock out mice and are going to analyze phenotypes. We consider that C4-hydroxylated sphinganine containing glycolipids play a significant role for maintaining functions of nutrient absorption.
저자
간행물 정보
발행기관
-
- 발행기관명
- 한국당과학회 [Korean Society for Glycoscience]
- 설립연도
- 2006
- 분야
- 의약학>약학
- 소개
- 본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.
간행물
-
- 간행물명
- 한국당과학회 학술대회
- 간기
- 연간
- 수록기간
- 2006~2022
- 십진분류
- KDC 517 DDC 614
이 권호 내 다른 논문 / 한국당과학회 학술대회 2008 Eastern Asian Glycoscience Symposium
피인용수 : 0건 (자료제공 : 네이버학술정보)
함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.
출처 : 네이버학술정보
0개의 논문이 장바구니에 담겼습니다.