노화 쥐의 골격근 유형에 따른 트레드밀 운동이 myostatin 및 follistatin 단백질 발현에 미치는 영향
Effect of exercise on myostatin and follistatin protein expression from the skeletal muscle types in aged rat
In the present study, the effects of treadmill exercise on expression of myostatin and follistatin protein expression of old aged rats according to skeletal muscle types. 16-month-old male rats(n=12) and male Sprague-Dwaley (n=12) were used as the old-aged groups. The Sprague-Dawley rats were randomly divided into 4 groups(n=6 in each group), as follows: the old-aged female group(n=6), the old-aged female and exercise group(n=6), the old-aged male group(n=6), old-aged male and exercise group(n=6). The rats in the exercise groups were forced to run on a motorized treadmill for 30 min once a day for 4 weeks. The load of exercise consisted of running at a speed of 2 m/min for the first 5 min, 5 m/min for the next 25 min, with a 0° inclination. The expression of myostatin in soleus muscle was lower in the old aged male rats compared with old aged female rats. In contrast, the expression of follistatin in soleus muscle was higher in the old aged male rats compared to old aged female rats. Treadmill exercise decreased expression of myostatin and, increased expression of follistatin in the soleus muscle. The expression of myostatin in gastrocnemius muscle was lower in the old aged female rats compared with old aged male rats. In contrast, the expression of follistatin in gastrocnemius muscle was not statistically significant. Treadmill exercise decreased expression of myostatin, however, the expression of follistatin was increased by treadmill exercise in the gastrocnemius muscle. Here in this study, we showed that treadmill exercise is a very useful strategy for preventing loss of muscle by inhibiting expression of myostatin and enhancing expression of follistatin.
목차
I. 서론 1. 연구의 필요성 2. 연구의 목적 II. 연구방법 1. 실험대상 2. 트레드밀 달리기 프로토콜 3. 웨스턴블롯팅 4. 자료처리 III. 결과 1. 가지미근의 myostatin과 follistatin 발현에 미치는 영향 2. 비복근의 myostatin과 follistatin 발현에 미치는 영향 IV. 논의 V. 결론 Acknowledgments 참고문헌 ABSTRACT
현대사회에 있어서 신체활동 연구의 주요 탐구과제는 ‘신체활동을 통한 교육’으로부터 ‘인간의 기본적인 움직임’을 목표로 하는 스포츠적인 움직임을 중심으로 한 생명 활동의 개념으로 급속히 변화되고 있는 추세이다. 이러한 환경변화에 대응하기 위하여 본학회는 ‘체육’적인 요소에서 ‘스포츠’적인 요소를 중심으로 한 학문적 정체성을 견고히 하고, 정기적인 학술지 발간과 학술활동을 통해 한국스포츠츠 문화의 발전과 학문적인 발전에 기여하는데 그 목적이 있다.