Dimethyl sulfoxide에 의한 세포내 칼슘이온 농도 증가가 안정적으로 형질 전환된 초파리 S2 세포에서 재조합 사람 cyclooxygenase 1의 발현에 미치는 영향
An Increased Intracellular Calcium Ion Concentration in Response to Dimethyl Sulfoxide Correlates with Enhanced Expression of Recombinant Human Cyclooxygenase 1 in Stably Transfected Drosophila melanogaster S2 Cells
Dimethyl sulfoxide (DMSO) increased the intracellular calcium ion concentration in stably transfected Drosophila melanogaster S2 cells expressing recombinant cyclooxygenase 1 (COX-1). DMSO did not increase the Drosophila NOS (dNOS) transcript level in calcium chelatortreated cells. Expression of recombinant COX-1 due to DMSO was diminished in cells treated with calcium chelators or channel blockers. Our results indicate that an increased intracellular calcium ion concentration due to DMSO is associated with up-regulation of the dNOS gene, leading to enhanced expression of COX-1.
목차
Abstract 1. Introduction 2. Materials and Methods 2.1. Cell culture 2.2. Measurement of intracellular calcium ion concentration 2.3. SDS-PAGE and Western blot analysis 2.4. Northern blot analysis 3. Results and Discussion 3.1. Effect of DMSO on expression of recombinant COX-1 from stably transfected S2 cells 3.2. Effect of DMSO on intracellular calcium ion concentration 3.3. Effect of the intracellular calcium ion concentration on up-regulation of the dNOS transcript by DMSO 3.4. Effect of calcium chelators on expression of recombinant COX-1 in DMSO-treated stably transfected S2 cells 3.5. Effect of calcium channel blockers on expression of recombinant COX-1 in DMSO-treated stably transfected S2 cells 4. Conclusions Acknowledgement References
키워드
recombinant cyclooxygenase 1dimethyl sulfoxideDrosophila melanogaster S2 cellscalcium ion
저자
장경화 [ Kyung Hwa Chang | Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University ]
박종화 [ Jong-Hwa Park | Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University ]
김도형 [ Do Hyung Kim | Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University ]
정하영 [ Ha Young Chung | Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University ]
황보전 [ Jeon Hwang-Bo | Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University ]
이현호 [ Hyun Ho Lee | Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University ]
이희영 [ Hee-Young Lee | Medican Co., Ltd., Seoul 135-891, Korea ]
손동화 [ Dong-Hwa Shon | Korea Food Research Institute ]
김원용 [ Wonyong Kim | Department of Microbiology & Research Institute for Translational System Biomics, Chung-Ang University College of Medicine ]
정인식 [ In Sik Chung | Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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