Earticle

초록

영어

The purpose of this study is to examine the growth inhibition of fungi or bacteria and the sterilizing capacity of wood vinegar a natural material to use it for the natural therapy. The wood vinegar is made from the liquid obtained by natural cooling of smoke coming out when the wood becomes the charcoal or by dry distillation of wood chips. For this study, 6 types of wood vinegar (sample-2, 2.2, 3, 4, 5 and 5-1) were prepared. Above wood vinegars had some effect to restrict the multiplication of athlete's foot fungi T. mentagrophytes & T. rubrum in the anti-fungus disk diffusion method, and the effect of sample-5(mechanical wood vinegar) was the best. In the anti-biotic disk diffusion method of above wood vinegars, all the wood vinegars showed the effect to restrict the multiplication of bacteria such as S. aureus, E. coli, A. baumannii, B. epidermidis and C. xerosis. Especially, sample-5 with the growth inhibition zone of 18-35mm size was the strongest anti-biotic. In the liquid medium of above wood vinegars, the minimum inhibitory concentration(MIC) of sample-2, 2.2, 3 and 4 against the athlete's foot fungus T. rubrum was 6.25%, and that of sample-5(mechanical type) was 1.5%. Thus, the inhibitory capacity of sample-5 was the best, too. The MIC of bacterial growth inhibition was 1.56% for S. aureus, E. coli, A. baumannii and B. epidermidis and 0.78% for C. xerosis, in which the effect of sample-5 was the best. The minimum bactericidal concentration(MBC) of sample-5 was 0.78% for S. aureus, C. xerosis and A. baumannii, and 1.56% for E. coli and B. epidermidis. Thus, its bactericidal power was the best among 6 wood vinegars. Wood vinegars sample-2 and 5 were solved in water, chloroform, hexane, butanol and ethyl acetate respectively and divided into separate fractions to treat above-mentioned 5 bacteria with them. As the result, all the samples showed the lethality. S. aureus showed the highest lethality in the chloroform fraction of sample-2 with 0.156 mg/ml of MIC, and its average lethality was high in order of ethyl acetate, chloroform, hexane. Sample-2 and 5 were mixed into chloroform and ethyl acetate to get 11 fractions. In MIC, fraction-4 had the highest lethality; its MBC was 0.156 mg/ml for S. aureus, 0.625 mg/ml for E. coli1, 0.156 mg/ml for A. baumannii, 0.156 mg/ml for B. epidermidis, and 0.078 mg/ml for C. xerosis. With the silica gel column, 11 fractions were obtained from sample-2. Among these fractions, the lines-2, 3, 4 and 5 of fraction-5 with high anti-biotic capacity were analyzed in Sephadex column chromatography. Then, after the MPLC treatment, they were analyzed with the ion spectra to find the single peak of 127 molecular weight. In conclusion, the wood vinegars may be a useful material for natural healing and microbial suppression on human body.

한국어

목초액을 음용하거나 무좀약 등으로 사용하는 예가 많으나 미생물학적 검정이 미비하여 본 연구에서는 무좀진균과 세균에 대하여 항균효과를 연구하였다. 항균성 검사는 디스크 확산법, 최소억제농도 측정, 최소살균농도측정, 용매를 이용한 목초액 분획들의 살균성을 측정하였고, chromatography, HPLC 및 mass spectrophotometer를 이용한 살균성 물질의 탐색을 하였다. 목초액 6종류(sample-2, 2-2, 3, 4, 5, 5-1)를 디스크확산법으로 측정한 결과는 무좀균 T.rubrum, T. mentagrophytes 및 세균 S. aureus, E. coli, A. baumannii, B. epidermidis, C. xerosis에서도 모든 목초액이 항 균성 증식억제를 보였다. 목초액 액체배지의 최소억제농도(MIC)와 최소살균농도(MBC) 측정에서는 T. rubrum은 1.5~6.25%였고, 세균은 0.78~1.56%였으며, sample-5가 가장 효과가 높았다. sample-2와 sample-5를 각각 물, chloroform, hexane, butanol, ethylacetate의 분획을 5종의 세균에게 처리한 결과 모두 특정 분획물들이 증식억제성을 나타냈다. sample-2의 chloroform 분획의 MIC 농도 0.156 mg/ml 로 가장 높은 치사성을 나타냈고, 평균적 치사성은 ethyl acetate, chloroform, hexane 순이었다. sample-2와 sample-5를 chloroform과 ethylacetate 혼합용매에서 분획물 11개의 분획을 얻었으며, MIC는 fraction-4가 치사성이 가장 높았으며, MBC는 0.078~0.156 mg/ml이었다. sample-2를 silica gel column을 통하여 11 fractions를 얻었으며, 이 분획들 중에서 항균성이 강한 fraction-5번의 lines-2, 3, 4, 5를 Sephadex column 크로마토그래피로 분석 후, 다시 MPLC 처리를 하고, Ion spectra로 분석하여 127분자량을 갖는 단일 peak를 발견하였다.

목차

초록
 1. 서론
 2. 재료 및 방법
  2.1 목초액의 선별
 3. 결과 및 고찰
  3.1 진균증식억제
  3.2 세균에 대한 디스크환산법에 의한 증식억제농도
  3.3 액체배지에서 최소억제 농도(MIC) 판정
 4. 결론
 참고문헌

키워드

목초액 무좀 세균 디스크 확산법 최소억제농도 최소살균농도 chloroform chromatography wood vinegar mycosis bacteria disc diffusion minimum inhibition concentration chloroform chromatography

저자

• 김유복 [ Kim Yubok | 동방대학원대학교 자연치유학과 ]
• 나선희 [ Sun Hee Na | 건국대학교 생명과학과 ]
• 김수영 [ Soo Young Kim | 건국대학교 생명과학과 ]
• 이형환 [ Hyung H. Lee | 동방대학원대학교 자연치유학과, 건국대학교 생명과학과 ] Corresponding author

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