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Analysis of Membrane Integrity and Mitochondrial Activity in Fresh and Cryopreserved Boar Sperm Using Flow Cytometry

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 29 No 4 (2005.12)바로가기
  • 페이지
    pp.253-257
  • 저자
    C. S. Park, Z. H. Li, N. D. Sung, D. I. Jin, P. Q. Cong, E. S. Kim, E. S. Song, Y. J. Yi
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A18779

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초록

영어
This study was carried out to evaluate the effects of washing medium, breed and washing temperature of fresh and frozen-thawed boar sperm on mitochondrial activity and membrane integrity by flow cytometry. More than 80% of fresh sperm washed with mTLP-PVA medium at 20℃ exhibited an intact membrane and a functional mitochondrion. With frozen-thawed samples, a large number of sperm showed both damaged membrane (36.4~46.9%) and nonfunctional mitochondrion (55.1~71.1%) in the mTLP-PVA and BTS washing media at 20℃. There were no breed effects of fresh and frozen-thawed sperm on mitochondrial activity and membrane integrity. The percentages of damaged membrane of fresh and frozen sperm, respectively, were higher at 4℃ washing temperature than at 20℃ washing temperature in the mTLP-PVA medium. We found that washing medium and washing temperature of fresh and frozen-thawed boar sperm were important for the analyses of mitochondrial activity and membrane integrity by flow cytometry.

목차

INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES

키워드

Membrane integrity Mitochondrial activity Boar sperm Flow cytometry

저자

  • C. S. Park [ Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University ]
  • Z. H. Li [ Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University ]
  • N. D. Sung [ Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University ]
  • D. I. Jin [ Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University ]
  • P. Q. Cong [ Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University ]
  • E. S. Kim [ Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University ]
  • E. S. Song [ Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University ]
  • Y. J. Yi [ Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 29 No 4

    피인용수 : 0(자료제공 : 네이버학술정보)

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