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Analysis of Membrane Integrity and Mitochondrial Activity in Fresh and Cryopreserved Boar Sperm Using Flow Cytometry

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 29 No 4 (2005.12)바로가기
  • 페이지
    pp.253-257
  • 저자
    C. S. Park, Z. H. Li, N. D. Sung, D. I. Jin, P. Q. Cong, E. S. Kim, E. S. Song, Y. J. Yi
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A18779

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초록

영어
This study was carried out to evaluate the effects of washing medium, breed and washing temperature of fresh and frozen-thawed boar sperm on mitochondrial activity and membrane integrity by flow cytometry. More than 80% of fresh sperm washed with mTLP-PVA medium at 20℃ exhibited an intact membrane and a functional mitochondrion. With frozen-thawed samples, a large number of sperm showed both damaged membrane (36.4~46.9%) and nonfunctional mitochondrion (55.1~71.1%) in the mTLP-PVA and BTS washing media at 20℃. There were no breed effects of fresh and frozen-thawed sperm on mitochondrial activity and membrane integrity. The percentages of damaged membrane of fresh and frozen sperm, respectively, were higher at 4℃ washing temperature than at 20℃ washing temperature in the mTLP-PVA medium. We found that washing medium and washing temperature of fresh and frozen-thawed boar sperm were important for the analyses of mitochondrial activity and membrane integrity by flow cytometry.

목차

INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES

저자

  • C. S. Park [ Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University ]
  • Z. H. Li [ Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University ]
  • N. D. Sung [ Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University ]
  • D. I. Jin [ Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University ]
  • P. Q. Cong [ Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University ]
  • E. S. Kim [ Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University ]
  • E. S. Song [ Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University ]
  • Y. J. Yi [ Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 29 No 4

    피인용수 : 0(자료제공 : 네이버학술정보)

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