Earticle

초록

영어

We were investigated for components depleted during a batch-culture of recombinant Chinese hamster ovary DG44 cells in chemically defined media. About after 5 days of cell cultured, cell growth and viability are usually reduced. It is predicted that specific components which help cell growth and antibody production are depleted in media. Therefore we investigated that rCHO-DG44 cell growth screening for the depletion components in Chemically-defined media. In the case of amino acid composition of the medium is analyzed by HPLC, but depletion or accumulation of other components are difficult to measure by analyzer. In this study, we collected supernatants of rCHO-DG44 cell culture in Chemically-defined media at day 4 and day 7 of culture. Then the each supernatant mixed with equal volume fresh media without specific components we wanted to monitor. rCHO-DG44 cell is cultured in mixed media. As a result, amino acids, vitamin and small molecules are depletion. By this method, we know depletion components for cell growth and antibody production. It is expected that it is useful for batch-culture enhance and fed-batch culture strategy. And the same method as above, other media and cell lines will be useful.

키워드

저자

• Eun-Ji LEE [ Department of Chemical Engineering, Han-yang University, Seoul, 133-791. ]
• Hong-Woo PARK [ Department of Chemical Engineering, Han-yang University, Seoul, 133-791. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576