In this study, we evaluated the efficiency of different adsorbents for the removal of plant-derived impurities during the pre-purification of paclitaxel from plant cell cultures. Using the synthetic adsorbents sylopute and active clay and their major components SiO2 and MgO, we performed adsorbent treatment and analyzed the paclitaxel precipitates recovered from hexane precipitation. When SiO2 was used, the highest purity (~58.1%) and yield (~91.5%) of paclitaxel were obtained. We also determined differences in the effectiveness of the adsorbent treatment according to changes in the surface area, pore volume and pore diameter of SiO2. Adsorbent treatment was more effective when pore diameter was larger (silica I [2.19 nm]
키워드
PaclitaxelSeparationPurificationAdsorbent
저자
Hyeon-Jeong OH [ Department of Chemical Engineering, Kongju National University, Cheonan 330-717, Korea. ]
Kyeong Youl JUNG [ Department of Chemical Engineering, Kongju National University, Cheonan 330-717, Korea. ]
Jin-Hyun KIM [ Department of Chemical Engineering, Kongju National University, Cheonan 330-717, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동