Gi-Hong JIN, Jun-Young KWON, Hyung-Jin NAM, Myoung-Sik KIM, Jun-Yong PARK, Sun-Hee JEONG, Yun-Young PAK, Min-Sub KIM, Hong-Yeol CHOI, Dong-Il KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A187512
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원문정보
초록
영어
Plant-derived peptones (PPs) have been utilized to improve cell growth and volumetric productivity in plant cell cultures. PPs contain amino acids, carbohydrates and minerals. Carbon (C) and nitrogen (N) sources have been known as major factors in plant cell cultures. Supplementation of chemically defined medium with PP was found to enhance cell growth and the production of human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) significantly in transgenic rice cell suspension cultures. Therefore, it was necessary to investigate the effects of C and N sources for the improvement of recombinant protein production. The concentrations of C and N sources used in this study were as follows: 0 (control), 1, 3, 5% (w/v) of sucrose and 10, 20, 30 g/L of PP. In case of adding 3% sucrose with 10 g/L of PP, maximum dry cell weight was 1.9-fold higher than that of control and the maximum level of hCTLA4Ig reached up to 64.8 mg/L. Whereas hCTLA4Ig was not produced in control cultures. In conclusion, it was found that the determination of optimal concentration ratio between C and N sources are important to improve cell growth and hCTLA4Ig production in transgenic rice cell cultures.
키워드
Rice cell cultureshCTLA4IgPlant-derived peptone
저자
Gi-Hong JIN [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동