We investigated certain components of the medium for cell growth and antibody production by grouping feeding conditions. The depletion of one or more essential nutrient resulted in cell death or reduced mAb production in batch culture. Therefore, the key for high performance of cell growth and mAb production is development of effective nutrient feeding strategies. This study evaluates the effect of grouped feeding conditions composed media on the growth rate and monoclonal antibody production. Feeding groups include Amino acids, Vitamins, Trace elements, Lipids, Other components and Growth factors. In the process, nutrient concentrations were maintained to avoid depletion of substances and glucose and/or glutamine supply to maintain proper concentration. The maximum viable cell density attained in the fed-batch culture was a 100% improvement over the control culture. The mAb titer is approximately 150% higher than in the batch culture. We have experimented a dihydrofolate reductase deficient Chinese Hamster Ovary DG44 Cells (rCHO-DG44) line in self made Chemicallydefined media. These feeding strategies will utilize available in other cells and media.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동