Earticle

초록

영어

In the past decade, many DNA mismatch detection methods have been proposed for analyzing SNPs (single nucleotide polymorphisms), which are one-base DNA sequence variations that are responsible for various inherited diseases and drug resistance. Among them, DNA ligase-based assay has been considered as one of the most potent methods in terms of its various applicability. However, DNA ligase-based methods for mismatch detection showed limitation due to its inevitable occurrence of false-positive result (observation of ligation product in G:T mismatch, that is serious disadvantage in cytosine/thymine(C/T)-type SNP analysis). In order to overcome the problem, we employed chemically-modified nucleobases, several universal or non-bonding nucleobases (such a 5-Nitroindole, Iso dG, Iso dC), at the 3’-end of a downstream ligation fragment and optimized successful DNA ligation reaction conditions for distinguishing G:C and G:T matches. In particular, the use of iso-dG and 5-nitroindole showed highly-distinctive detection results of C/T type SNP in DNA ligase-based analysis. The developed DNA-ligation methods using modified-end DNA oligomer as ligation fragment will be usefully employed for development of highly-accurate system of SNP analysis.

키워드

저자

• Eui Kyoung JANG [ Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam 339-700, Korea. ]
• Ki Baek YEO [ Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam 339-700, Korea. ]
• Mi Ran KI [ Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam 339-700, Korea. ]
• Ki Ha MIN [ Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam 339-700, Korea. ]
• Seung Pil PACK [ Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam 339-700, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576