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Our previous study demonstrated that some polyphenols could stop neuronal SNARE zippering [1]. Membrane fusion intermediates such as hemifusion and partially zipped SNARE complexes, which are otherwise transient, could be captured and dissected by the identified polyphenols [1]. The polyphenols did indeed impair neuroexocytosis by inhibiting SNARE complex formation in neuron and paralyzed muscles [2]. Based on these results, we hypothesized that some polyphenols interfere with SNARE complex formation in cells other than neuronal cells, though the functional SNARE complex-comprising proteins might be different. Among the various cells in which SNARE proteins are involved in trafficking pathways and exocytosis, mast cell was selected to demonstrate our hypothesis, as the secretory behaviors of mast cells have been relatively well-characterized. Here, it was demonstrated that polyphenols inhibited SNARE complex formation in mast cells in a SNARE-specific manner, which might provide a clue to how polyphenols exhibit anti-allergic activity. Polyphenols regulated SNARE complex formation in mast cells, resulting in degranulation inhibition. As a result of selective or common binding of polyphenols to two different SNARE complexes, membrane fusion of the corresponding granules with plasma membrane was selectively or commonly inhibited, leading to polyphenols’ differential degranulation inhibition of distinct subsets of granules. Because all intracellular membrane fusion events are mediated by SNARE-family members and SNARE proteins are distributed on nearly every membrane of all eukaryotic cells, our results implicate the possibility that formation of a variety of SNARE complexes in numerous cell types is controlled by polyphenols, which, in turn, might regulate corresponding membrane trafficking.

키워드

저자

• Dae-Hyuk KWEON [ Dept. of Genetic Engineering, Sungkyunkwan University, Suwon, 440-746. ]
• Yoosoo YANG [ Dept. of Genetic Engineering, Sungkyunkwan University, Suwon, 440-746. ]
• Haul HEO [ Dept. of Genetic Engineering, Sungkyunkwan University, Suwon, 440-746. ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576