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Development of Non-self Immunogenic Mucosal Adjuvant for sublingual delivery of vaccines
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- 발행기관
- 한국생물공학회 바로가기
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- 간행물
- 한국생물공학회 학술대회 바로가기
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- 통권
- 2012 추계학술대회 (2012.09)바로가기
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- 페이지
- pp.27-27
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A187476
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
- 영어
- Backgrounds / Objectives: Cholera toxin (CT) is a strong systemic and mucosal adjuvant that greatly enhances IgG, IgA and cellular immune responses. The activity of CT as a mucosal adjuvant has been confirmed with a variety of antigens. The adjuvant properties of CT have been studied in animal models using both in vivo and in vitro experiments. However, CT is a strong enterotoxin. It was known that oral administration of 10ug of CT is sufficient to induce significant diarrhea in humans suggesting that toxicity may limit the clinical use of CT.
Experimentals / Methods: CT A1 subunit (CTA1) was genetically fused with protein transduction domain (PTD) which has been known to deliver fused protein highly efficiently into the cells after non-specific binding to the cell surface. To enhance the cellular uptake, we attached PTD at both N-and C- termini of CTA1 (TCTA1T) and analyzed feasibility of TCTA1T as an adjuvant on humoral and cellular immune response.
Results: Intranasal delivery of recombinant TCTA1T which was purified from Escherichia coli with ovalbumin (OVA) significantly augmented OVA-specific both systemic and mucosal immune responses which were comparable to that of CT. The adjuvant activity of TCTA1T was enzyme dependent, because the immune response induced by enzymatically inactive TmCTA1T was significantly lower than that of TCTA1T. It also dramatically increased the number of OVA-specific antibody secreting cells and in vivo CTL activity in the lung and spleen. In addition, mucosal administration of TCTA1T resulted in an increase of mucosa-derived dendritic cells in draining lymph node, which is similar to native CT. However, in contrast to CT, TCTAlT was not either reactogenic or toxic in mice and its ADP-ribosyltransferase activity was much lower than that of CT (≈ 5%).
Conclusions: Our results demonstrate that non-targeted delivery of enzymatic active TCTA1 is non-toxic and has immune-modulating activity, suggesting a new approach to develop a safe and effective mucosal adjuvant.
저자
간행물 정보
발행기관
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- 발행기관명
- 한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
- 설립연도
- 1984
- 분야
- 공학>생물공학
- 소개
- 이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다 1. 생물공학 분야의 발전을 위한 연구 협력 2. 생물공학의 실용화를 촉진시키기 위한 산학 협동 3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최 4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간 5. 생물공학 발전을 위한 정책 건의 6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
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- 간행물명
- 한국생물공학회 학술대회
- 간기
- 반년간
- 수록기간
- 1985~2013
- 십진분류
- KDC 476 DDC 576
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