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Effect of Inhibitor of Glycogen Synthase Kinase 3 on Self-Renewal of Human Embryonic Stem Cells

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 29 No 2 (2005.06)바로가기
  • 페이지
    pp.93-99
  • 저자
    Lee Eunyoung, Rho Jeung-yon, Yu Kwon, Paik Sang-Gi, Lee Kyung-Kwang, Han Yong-Mahn
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A18713

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원문정보

초록

영어
Human embryonic stem cells (hESCs) derived from the inner cell mass of blastocysts have the ability to renew themselves and to differentiate into cell types of all lineage. The present study was carried out to investigate whether the Wnt signaling pathway is related to maintaining self-renewal of hESCs. Glycogen Synthase Kinase 3 (GSK-3) inhibitor, BIO ((2''Z,3''E)-6-Bromoindirubin-3''-oxime) was treated to Miz-hES1 line for activation of Wnt signaling pathway. BIO-nontreated hESCs (control) and BID-treated hESCs were cultured for 5 days in the modified feeder-free system. During the culture of hESCs, differences were observed in the colony morphology between 2 groups. Controls were spread outwards whereas BIO-nontreated hESCs were clumped in the center and the differentiated cells were spreading outwards in the edges. The results of stem cell specific marker staining indicated that control were differentiated in large part whereas BIO-treated hESCs maintain self-renewal in the center of the colony. The results of lineage marker staining suggested that outer cells of the hESC colony were differentiated to the neuronal progenitor cells in both control and BIO-treated hESC. These results indicate that Wnt signaling is related to self-renewal in hESCs. In addition, control group showed higher composition of apoptotic cells $(23.76\%)$ than the BID-treated group $(5.59\%)$. These results indicate that BIO is effective on antapoptosis of hESCs.

목차

INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES

키워드

Human embryonic stem cells Wnt signaling pathway BIO Self-renewal Antiapoptosis

저자

  • Lee Eunyoung [ Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology(KRIBB) ]
  • Rho Jeung-yon [ Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology(KRIBB) ]
  • Yu Kwon [ Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology(KRIBB) ]
  • Paik Sang-Gi [ Department of Biology, Chungnam National University ]
  • Lee Kyung-Kwang [ Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology(KRIBB) ]
  • Han Yong-Mahn [ Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology(KRIBB) ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

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