Chen J. B., Sa S. J., Cao Y., Choi S. H., Cheong H. T., Yang B. K., Park C. K.
언어
영어(ENG)
URL
https://www.earticle.net/Article/A18711
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4,000원
원문정보
초록
영어
This study were examined whether plasminogen activators (PAs) are produced by porcine fresh or frozen-thawed cumulus-oocytes complexes (COCs) and cumulus cell free-oocytes. In fresh or frozen-thawed COCs and oocytes for 0 hour cultured, no activity of PAs was detected. However, at 24 hours of culture urokinase-type plasminogen activator (uPA) was detected in COCs and denuded oocytes. In the frozen-thawed COCs and cumulus cell free-oocytes cultured for 24 hours, no PAs were observed. After COCs were cultured for 48 hours, tissue-type plasminogen activator (tPA) and tPA-PAI were observed in COCs only. In the frozen-thawed COCs and cumulus cell free-oocytes cultured for 48 hours, no PAs were observed. These results suggest that uPA, tPA and tPA-PAI are produced by porcine COCs, but only uPA by oocytes during maturation for 24 hours. Only tPA, and tPA-PAI are produced by COCs cultured for 48 hours, and no PAs are produced by denuded-oocytes cultured for 48 hours. In all of the frozen-thawed groups, no PAs are observed by COCs and denuded-oocytes.
목차
INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES
키워드
In vitro maturationPigFrozen-thawed oocytesPlasminogen activators
저자
Chen J. B. [ College of Animal Resources Science, Kangwon National University ]
Sa S. J. [ College of Animal Resources Science, Kangwon National University ]
Cao Y. [ College of Bioengineering, Dalian University ]
Choi S. H. [ Animal Genetic Resources Station, National Livestock Research Institute ]
Cheong H. T. [ College of Animal Resources Science, Kangwon National University ]
Yang B. K. [ College of Animal Resources Science, Kangwon National University ]
Park C. K. [ Division of Animal Resourcem Kangwon National University ]