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Effects of Various Addition and Exclusion Time of Glucose on Development of Mouse Two-Cell Embryos

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 28 No 4 (2004.12)바로가기
  • 페이지
    pp.227-233
  • 저자
    Park, S. B., Park, K. S., Lee, T. H., Chun, S. S., Kim, K. S., Song, H. B.
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A18690

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원문정보

초록

영어
This study was conducted to investigate the effect of various addition and exclusion time of glucose (Control: no addition, A: 24～72 h, B: 24～48 h, C: 48～72 h, D: 0～72 h, E: 0～48 h, F: 0～24 h and 48～72 h, G: 0～24 h) on embryonic developmental capacity of 2-cell embryos in mice. Developed blastocysts were assessed for mean cell number by differential staining. The zona-intact blastocyst (ZiB) rates were higher (p<0.05) in group B than control. However, the zona-escape blastocyst (ZeB) rates were not significantly different in all groups. At 72 h, total blastocyst (ZiB + ZeB) formation rates were not significantly different in all groups. The mean cell number was not significantly different among all groups. The inner cell mass (ICM) cell number was higher (p<0.05) in group F than control, group A, B and G. The trophectoderm (TE) cell number was higher (p<0.05) in control than group A and D. The %ICM was higher (p<0.05) in group C, D and F than control. The ICM : TE ratio was not significantly different in all groups. Between control and glucose group, no significant difference was observed in the total blastocysts (ZiB + ZeB) formation rates. Also, no significant difference was observed in the mean cell number, ICM cell number and ICM : TE ratio. However the TE cell number was higher (p<0.05) in control than glucose group and %ICM was higher (p<0.05) in glucose group than control. In conclusion, glucose added in culture medium was not inhibitory on blastocyst formation but glucose added for 48 ～72 h in culture medium increases %ICM of blastocysts in mice.

목차

INTRODUCTION
 MATERIALS AND METHODS
  Preparation of Media
  Prepatation of hFF
  Prepatation of Two Cell Embryos
  Culture of Embryos
  Differentially Staining of Blastocysts
  Observation
  Satistical Analysis
  Photographs
 RESULTS
 DISCUSSION
 REFERENCES

키워드

Blastocyst Glucose Cell numbers Mouse 2-cell embryo Inner cell mass

저자

  • Park, S. B. [ Department of Animal Science, Daegu University ]
  • Park, K. S. [ Department of Obstetrics and Gynecology, Kyungpook National University Hospital ]
  • Lee, T. H. [ Department of Obstetrics and Gynecology, Kyungpook National University Hospital ]
  • Chun, S. S. [ Department of Obstetrics and Gynecology, Kyungpook National University Hospital ]
  • Kim, K. S. [ Cheonan Yonam College ]
  • Song, H. B. [ Department of Animal Science, Daegu University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

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