Earticle

초록

영어

The shortage of human organs for transplantation has induced the research on the possibility of using animal as porcine. However, pig to human transplantation as known as xeno-transplantation has major problem as immunorejection. Recently, the solutions of pig to human xenotransplantation are commonly mentioned as having a genetically modification which include alpha 1, 3 galatosyl transferase knockout (GTKO) and immune-suppressing gene transgenic model. Unfortunately, the expression level of transgenic gene is very low activity. Therefore, development of gene overexpression system is the most urgent issue. Also, the tissue specific overexpression system is very important. Because most blood vessels are endothelial cells, establishment of the endothelial-specific promoter is attractive candidates for the introduction of suppressing immunorejection. In this study, we focus the ICAM2 promoter which has endothelial-specific regulatory region. To detect the regulatory region of ICAM2 promoter, we cloned 3.7 kb size mini-pig ICAM2 promoter. We conduct serial deletion of 5' flanking region of mini-pig ICAM2 promoter then selected promoter size as 1 kb, 1.5 kb, 2 kb, 2.5 kb, and 3 kb. To analyze promoter activity, luciferase assay system was conducted among these vectors and compare endothelial activity with epithelial cells. The reporter gene assay revealed that ICAM2 promoter has critical activity in endothelial cells (CPAE) and 1 kb size of ICAM2 promoter activity was significantly increased. Taken together, our studies suggest that mini-pig ICMA2 promoter is endothelial cell specific overexpression promoter and among above all size of promoters, 1 kb size promoter is optimal candidate to overcome the vascular immunorejection in pig to human xenotransplantation.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Cell Culture
  Construction of Luciferase Reporter Gene Vectors
  Sequence Analysis
  Transfections and Analysis of Promoter Activity
  Expression Vector Construction of 1 kb Size Promoter
  Transfections and Western Blot Analysis
  Immnunocytochemistry
 RESULT
 Cloning of Mini-pig ICAM2 Promoter
 DISCUSSION
 REFERENCES

키워드

저자

• Hoon Jang [ Regenerative Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea, Functional Genomics, School of Engineering, University of Science and Technology (UST), Daejeon 305-350, Republic of Korea ]
• Won-Gu Jang [ Regenerative Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea ]
• Dong Un Kim [ lRe용enerative Medicine Research Center, Korea Research Institute 01 Bioscience and Biotechnology, Da에eon 305-806, Republic 01 Korea ]
• Eun-Jung Kim [ lRe용enerative Medicine Research Center, Korea Research Institute 01 Bioscience and Biotechnology, Da에eon 305-806, Republic 01 Korea ]
• Sung Soo Hwang [ Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Republic of Korea ]
• Keon Bong Oh [ 3 Animal Biotechnology Division, National Institute 01 Animal Science, Rural Development Administration, Suwon 441-706, Republic 01 Korea ]
• Jeong-Woong Lee [ Regenerative Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea, Functional Genomics, School of Engineering, University of Science and Technology (UST), Daejeon 305-350, Republic of Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636