번식한우 사양관리(비육전후)에 따른 지방산결합단백질 4, 5(FABP4, 5) 유전자와 육질의 연관성 분석
Association of Microsatellite Marker in FABP4,5 Gene with Marbling Score and Feeding and Management in Breed Hanwoo
The bovine fatty acid binding protein 4 and 5 (FABP4 and 5) is a major positional and physiological candidate gene for the bovine marbling and carcass weight. The aim of this study was to evaluate the association between economic traits of Korean cattle (Hanwoo) and genetic variation in fatty acid binding protein 4 and 5 (FABP4 and 5) genes within carcass/meat quality traits and the before/after of fatting in breed Hanwoo. Here, we characterized the nucleotide polymorphism of FABP4 and 5 in 86 cattle. We were detected the variability of three types (GG, AG, and AA) by PCR, and economic traits were analyzed by the mixed regression model implemented in the ASReml program. As the result of statistical and supersonic analysis, FABP4 gene was highly showed significant effect (p<0.006) on marbling score (MS), in contrast FABP5 gene was lowed (p<0.084) on MS before fatting. But, FABP4 gene was highly showed significant effect (p<0.0054) on MS, in contrast FABP5 gene lowest (p<0.0899) on MS in the after of fatting. Compare to supersonic result before fatting in FABP4 gene, it was detected type GG: (p<7.18), AG: (p<8.50), and AA: (p<10.50) (n=50), showed type GG: (p<4.88), AG: (p<2.33), and AA: (p<0.00) after weed out (n=20). Futhermore, it was detected type GG: (p<9.30), AG: (p<7.95), and AA: (p<7.40) (n=50) before fatting in the FABP5 gene. It was shown type GG: (p<2.67), AG: (p<3.50), and AA: (p<5.00) after weed out (n=50). Our results indicate that FABP4 and 5 gene transcription is regulated by the environment of feeding and management, and suggest that feeding and management could be potential key in determining FABP4 and 5 genes transcription for carcass/meat quality traits in breed Hanwoo.
목차
ABSTRACT 서론 재료 및 방법 공시재료 및 Genomic DNA 추출 PCR-RFLP을 이용한 개체벌 유전자형 판정 경제형질과의 통계적 관련성 분석 결과 FABP4,5 유전자형 분석 고찰 인용문헌
키워드
Breed HanwooFABP4FABP5Marbling score
저자
김봉순 [ Bong-Sun Kim | 안성시 농업기술센터 ]
장길원 [ Kil-Won Chang | 농촌진흥청 축산과학원 ]
이승환 [ Seung-Hwan Lee | 농촌진흥청 축산과학원 ]
정학재 [ Hak-Jac Chung | 농촌진흥청 축산과학원 ]
양보석 [ Bo-Suk Yang | 농촌진흥청 축산과학원 ]
박진기 [ Jin-ki Park | 농촌진흥청 축산과학원 ]
김민수 [ Min-Su Kim | 한경대학교 동물생명과학과 ]
임선화 [ Seon-Hwa Lim | 한경대학교 동물생명과학과 ]
박채원 [ Chae-Won Park | 한경대학교 동물생명과학과 ]
민관식 [ Kwan-Sik Min | 한경대학교 동물생명과학과 ]
양병철 [ Byoung-Chul Yang | 농촌진흥청 축산과학원 ]
Corresponding Author