In mammals, male and female germline stem cells are derived from primodial germ cells. Despite many efforts to identify stem cells from gonads, there has been little successe to identify germline stem cells yet. In this study, we isolate and characterized porcine germline stem cells using only stem cell markers that are prevalently expressed in various tissues. Gonadal cells derived from both male and female formed colonies and showed AP activities and different lectin binding properties. Pluripotency of germline stem cells was also identified by positive signals against putative stem cells markers such as SSEA-1 and SSEA-3. In addition, nestin was also found in primary gonad cells that have a similar morphology to the AP-positive cells. The nestin expression suggests that the germline stem cells may have similar expression of the prevalent stem cell markers found in other tissues. The demonstration of nestin expression together with pluripotent cell markers calls further investigation of the possible differentiation of nestin-positive cells into neurons.
목차
I. INTRODUCTION II. MATERIALS AND METHODS 1. Animals and primary cell culture 2. Immunocytochemical analysis and Lectin binding 3. Alkaline phosphatase staining III. RESULTS 1. General appearance of E45 fetuses from pregnant pigs 2. Colonies of germline stem cells showed activity of alkakine phosphatase 3. Specific stem cell marker-positive germline stem cells 4. Highly expressed glycoproteins on the germline stem cells IV. DISCUSSION V. REFERENCES
키워드
PorcineGermline stem cellsSSEA-1;SSEA-3Nestin
저자
최승철 [ Choi, S. C. | School of Life Sciences and Biotechnology, Korea University ]
연홍희 [ H. H. Yeon | School of Life Sciences and Biotechnology, Korea University ]
최승규 [ S. K. Choi | School of Life Sciences and Biotechnology, Korea University ]
이황 [ H. Lee | School of Life Sciences and Biotechnology, Korea University ]
홍수 [ S. Hong | School of Life Sciences and Biotechnology, Korea University ]
박창식 [ C. S. Park | Division of Animal Science & Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University ]
이성호 [ S. H. Lee | Kongju University ]
이상호 [ S. H. Lee | School of Life Sciences and Biotechnology, Korea University ]