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Study on the Factors Influencing Fertilization and Developmental Rate of in vitro Cultured Cat Oocytes Recovered from Ovaries Collected at Different Stages of the Reproductive Cycle
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- 발행기관
- 한국동물생명공학회(구 한국동물번식학회) 바로가기
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- 간행물
- Reproductive & developmental biology 바로가기
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- 통권
- Volume 28 No 1 (2004.03)바로가기
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- 페이지
- pp.53-57
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A18641
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원문정보
초록
- 영어
- The study was carried out to investigate the effects of morphology, reproductive cycle, incubation time and activation of oocytes on in vitro maturation of cat oocytes and development of IVM/IVF embryos. The results were summarized as follows: 1. When recovered from ovaries collected at different stages of the reproductive cycle (inactive, follicular and luteal stage), the developmental rates of oocytes to GV and MI stage were 72.5% and 27.5%, 57.5% and 7.5%, 62.5% and 17.5%, respectively. 2. The developmental rates of oocytes with cumulus cells to GV and MI stage in different conditions of incubation (5% CO₂, 95% O₂ and 10% CO₂, 90% O₂) were 70.0% and 27.5%, 52.5% and 20.0%, 55.0% and 12.5%, respectively. 3. The developmental rates to GV and MI oocytes when cultured at different time of incubation (17∼20, 21∼24, 25∼28 and 29∼32 h) were 67.5% and 20.0%, 67.5% and 30.0%, 62.5% and 22.5%, 65.0% and 15.0%, respectively. 4. The fertilization and cleavage rates of freshly collected oocytes with and without cumulus cells were 72.5% and 25.0%, 37.5% and 7.5%, respectively. The rates were greater in oocytes with cumulus cells than those without cumulus cells. 5. The fertilization and cleavage rates of oocytes recovered from ovaries collected at different stages of the reproductive cycle (inactive, follicular and luteal stage) were 75.0% and 25.0%, 40.0% and 7.5%, 50.0% and 15.0%, respectively.
목차
I. INTRODUCTION
II. MATERIALS AND METHODS
1. Oocyte recovery and culture
2. Pretreatment of sperm
3. In vitro gertilization
4. Assessment of fertilization and developmental rate
5. Statistical analysis
III. RESULTS AND DISCUSSION
1. Developmental rates of ovum collected at different reproductive stages
2. Developmental rates at different culture conditions
3. IVF time and fertilization rate
4. The relationship between morphology and developmental rate
5. The relationship between fertilization rate and reproductive cycles
IV. REFERENCES
키워드
Cat;Developmental rate Reproductive cycle Incubation time저자
간행물 정보
발행기관
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- 발행기관명
- 한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
- 설립연도
- 1976
- 분야
- 농수해양>축산학
- 소개
- 동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.
간행물
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- 간행물명
- Reproductive & developmental biology
- 간기
- 계간
- pISSN
- 1738-2432
- eISSN
- 2288-0151
- 수록기간
- 1977~2018
- 십진분류
- KDC 527 DDC 636
이 권호 내 다른 논문 / Reproductive & developmental biology Volume 28 No 1
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