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[Poster Presentation] - Reprogramming / Epigenetic

Differential Regulation of Reprogramming Transcription Factors in P19 Cells

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 36 No 2 Supplement (2012.06)바로가기
  • 페이지
    pp.125-125
  • 저자
    Byoung-Chul Yang, Hee Kyoung Chung, Hak-Jae Chung, Kyung-Woon Kim, Deug-Woo Han, Jin-Hee An, Myoung-Seob Choi, Jin-Ki Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A178380

원문정보

초록

영어
Oct4 and Nanog are transcription factors involved in pluripotency of stem cells. In general, Oct4 is up-regulated by Nanog. In previous results, however, Oct4 was differentially regulated by various doses of Nanog in P19 cells. High dose Nanog down-regulated the Oct4 expression. This negative feedback event was performed by DNMT and HDAC through the inhibitor assays (5-AZA-cytidine and trichostatin A). To identify the precise recruited sites for DNMT and HDAC, ChIP assay was performed in differential doses of Nanog. As a result, HDAC1, HDAC2, DNMT3A and Nanog were recruited to CR2, CR3, CR1, and CR4 upon high dose Nanog, respectively. Next, to investigate the differentiation potency of the cells upon high dose Nanog, RT-PCR with specific markers for three germ layers was performed. However, there was no increase for three germ layers in high dose Nanog treated cells except E-cadherin expression. E-cadherin is a specific marker for epithelial cells. Taken together, high dose Nanog induces Oct4 down-regulation and results in differentiating embryonic carcinoma cells to epithelial cell type. These results will be helpful for study on regulation of pluripotency-related genes in embryonic stem cells. * This study was supported by 2012 Post Doctoral Fellowship Program of National Institute of Animal Science, Rural Development Administration, Republic of Korea. This work received grant support from the Agenda Program (No.PJ007577), Rural Development Administration, Republic of Korea.

키워드

Oct4 Nanog Negative feedback

저자

  • Byoung-Chul Yang [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Republic of Korea ]
  • Hee Kyoung Chung [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Republic of Korea ]
  • Hak-Jae Chung [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Republic of Korea ]
  • Kyung-Woon Kim [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Republic of Korea ]
  • Deug-Woo Han [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Republic of Korea ]
  • Jin-Hee An [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Republic of Korea ]
  • Myoung-Seob Choi [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Republic of Korea ]
  • Jin-Ki Park [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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