Earticle

초록

영어

Ski protein is a nuclear transcription factor that does not bind DNA directly. Due to its unique binding properties with multiple factors, Ski could perform various roles in the regulation of both cellular proliferation and differentiation. We had previously reported that Ski protein is present in granulosa cells of atretic follicles, but not in preovulatory follicles, suggesting that Ski has a role in apoptosis of granulosa cells. The alternative fate of granulosa cells other than apoptosis is to differentiate to luteal cells; however, it is unknown whether Ski is expressed and has a role in granulosa cells undergoing luteinization. Thus, the aim of the present study was to examine whether the initiation of luteinization with luteinizing hormone (LH) directly regulates expression of Ski in the luteinized granulosa and luteal cells after ovulation by in vitro models. RT-PCR and real time PCR analysis respectively revealed that LH had no effect on c-Ski mRNA expression in the cultured granulosa cells regardless of LH treatment. Though Ski protein isabsent in granulosa cells of preovulatory follicle, its mRNA(c-Ski) was expressed and the level was unchanged even after LH surge. Taken together, these results demonstrated that Ski protein expression is induced in granulosa cells upon luteinization, and suggested that its expression is regulated post-transcriptionally. Moreover, expression of mRNA of Arkadia, an E3 ubiquitin ligases, in luteinizing granulosa cells in vivo was assessed by real time-PCR. The levels of Arkadia mRNA expression were unchanged during follicular growth and post ovulatory luteinization. These findings suggest that Ski protein level may be regulated during luteinization at translational and/or post-translational level but not by Arkadia.

키워드

저자

• Dan-Bi Shin [ Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea ]
• Dong Hun Kim [ Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea ]
• Soo Bong Park [ Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea ]
• Yeoung-Gyu Ko [ Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea ]
• Sung Woo Kim [ Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea ]
• Yoon Jung Do [ Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea ]
• Hyun Kim [ Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan, Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea ]

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간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & Developmental Biology(Supplement)

• 간기

  연간

• 수록기간

  2001~2017

• 십진분류

  KDC 527 DDC 636