Earticle

초록

영어

Sloan-Kettering virus gene product of a cellular pro-oncogene c-Ski is an unique nuclear pro-onco protein and belongs to the Ski/Sno proto-oncogene family. Ski plays multiple roles in a variety of cell types, it can induce both oncogenic transformation and terminal muscle differentiation when expressed at high levels. Ski protein is implicated in proliferation/differentiation in a variety of cells. The alternative fate of granulosa cells other than apoptosis is to differentiate to luteal cells, however, it is unknown whether Ski is expressed and has a role in granulosa cells undergoing luteinization. Thus, the aim of this study was, by means of immunohistochemical methods, to locate Ski protein in the rat ovaries during ovulation and corpora lutea(CL) formation to predict the possible involvement of Ski in luteinization. In addition, to examine whether the initiation of luteinization with luteinizing hormone(LH) directly regulates expression of Ski in the luteinized granulosa and luteal cells after ovulation by in vivo models. In order to examine the expression pattern of Ski protein along with the progress of luteinization, follicular growth was induced by administration of equine chorionic gonadtropin to immature female rat, and luteinization was induced by human chorionic gonadtropin treatment to mimic luteinizing hormone(LH) surge. While no Ski-positive granulosa cells were present in preovulatory follicle, Ski protein expression was induced in response to LH surge, and was maintained after the formation of corpus luteum(CL). These results indicate that Ski is profoundly expressed in the luteinized granulosa cells and luteal cells of CL during luteinization, and suggest that Ski may play a role in luteinization of granulosa cells.

키워드

저자

• Eun-Ji Park [ Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea ]
• Dong Hun Kim [ Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea ]
• Soo Bong Park [ Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea ]
• Yeoung-Gyu Ko [ Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea ]
• Sung Woo Kim [ Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea ]
• Yoon Jung Do [ Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea ]
• Hyun Kim [ Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences,The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan, Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & Developmental Biology(Supplement)

• 간기

  연간

• 수록기간

  2001~2017

• 십진분류

  KDC 527 DDC 636