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[Poster Presentation] - Oocyte Maturation / Embryonic Development

The Participation of BMP15 and GDF9 Signaling Pathways in Mediating Porcine Oocyte Maturation In Vitro

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 36 No 2 Supplement (2012.06)바로가기
  • 페이지
    pp.105-105
  • 저자
    Qing-Ling Wang, Shao-Chen Sun, Xing-Wei Liang, Sung-Hyun Lee, Nam-Hyung Kim, Xiang-Shun Cui
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A178365

원문정보

초록

영어
It is well established that mammalian cumulus cell (CC) expansion requires BMP15 (bone morphogenetic protein bone morphogenetic protein 15) and GDF9 (growth differentiation factor 9). However, the mechanisms of the factors in CC expansion are largely unclear. This study was conducted to examine the two paracrine factors and their receptor SMAD intracellular signaling mechanism of mediating porcine CC expansion and oocyte maturation, and to compare COCs (Cumulus–oocyte complexes) maturation to DOs (Denuded oocytes). COCs and DOs were in vitro matured in medium with FSH, LH and TGFB superfamily antagonists. Our results showed that the expansion of COCs was unaffected by addition of GDF9 and BMP15 recombinant protein, but cumulus cell proliferation and DOs maturation rate were enhanced. The mRNA expressions of SMAD receptor confirmed that oocytes secreted factors that activate SMAD3,4 and SMAD1 in granulosa cells and oocytes, but unaffected SMAD2. Treatment of COCs with a SMAD2/3 phosphorylation inhibitor (SB431542) inhibited CC expansion and expression of TNFAIP6. SB431542 also was revealed to inhibit DOs maturation. The activation of CC SMAD signaling by oocytes, and the requirement of SMAD2/3 signaling for expansion and oocyte maturation were studied in pig. Nonetheless, porcine oocyte maturation without SMAD2/3 signaling is likely to be needed for optimal matrix formation, but also BMP15 and GDF9 is likely to be needed in oocyte.

키워드

BMP15 GDF9 SMAD COC Oocyte maturation

저자

  • Qing-Ling Wang [ Department of Animal Sciences, Chungbuk National University ]
  • Shao-Chen Sun [ Department of Animal Sciences, Chungbuk National University ]
  • Xing-Wei Liang [ Department of Animal Sciences, Chungbuk National University ]
  • Sung-Hyun Lee [ Department of Animal Sciences, Chungbuk National University ]
  • Nam-Hyung Kim [ Department of Animal Sciences, Chungbuk National University ]
  • Xiang-Shun Cui [ Department of Animal Sciences, Chungbuk National University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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