Earticle

초록

영어

The coupling of autophagy and endoplasmic reticulum (ER) stress has been implicated in a variety of biological processes. However, little is known regarding the involvement of the autophagy/ER stress pathway in early embryogenesis or the underlying mechanism (s). Here, we showed that the developmental competence of in vitro-produced (IVP) bovine embryos was highly dependent on the autophagy/ER stress balance. Although relative abundances of autophagy-associated gene transcripts, including LC3, Atg5, and Atg7 transcripts, were high in oocytes and throughout the early stages of preattachment development, extensive autophagosome formation was only detected in fertilized embryos. Using inducer and inhibitor of autophagy, we showed that transient elevation of autophagic activity during early preattachment development greatly increased the blastocyst development rate, trophectoderm cell numbers, and blastomere survival; these same parameters were reduced by both inhibition and prolonged induction of autophagy. Interestingly, the induction of autophagy reduced ER stress and associated damage, while the developmental defects in autophagy-inhibited embryos were significantly alleviated by ER stress inhibitor treatment, indicating that autophagy is a negative regulator of ER stress inearly embryos. Collectively, these results suggest that early embryo genesis of IVP bovine embryos depends on an appropriate balance between autophagy and ER stress. These findings may increase our understanding of important early developmental events by providing compelling evidence concerning the tight association between autophagy and ER stress, and may contribute to the development of strategies for the production of IVP bovine blastocysts with high developmental competence.

키워드

저자

• Bong-Seok Song [ National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk ]
• Seung-Bin Yoon [ National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk ]
• Ji-Su Kim [ National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk ]
• Bo-Woong Sim [ National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk ]
• Young-Hyun Kim [ National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk, Department of Functional Genomics, University of Science and Technology, Daejeon 305-333, Republic of Korea ]
• Jae-Jin Cha [ National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk ]
• Seon-A Choi [ National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk ]
• Sun-Uk Kim [ National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk, Department of Functional Genomics, University of Science and Technology, Daejeon 305-333, Republic of Korea ]
• Kyu-Tae Chang [ National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk, Department of Functional Genomics, University of Science and Technology, Daejeon 305-333, Republic of Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & Developmental Biology(Supplement)

• 간기

  연간

• 수록기간

  2001~2017

• 십진분류

  KDC 527 DDC 636