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[Poster Presentation] - Oocyte Maturation / Embryonic Development

Effect of Porcine Oocyte Chromatin Configuration on the Maturation and Development Potential

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 36 No 2 Supplement (2012.06)바로가기
  • 페이지
    pp.87-87
  • 저자
    Min-Gu Lee, Jin-Yu Zhang, Rong-Xun Han, Yun-Fei Diao, Reza K. Oqani, Dong-Il Jin
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A178348

원문정보

초록

영어
In all the studies of mammalian species, chromatin in the germinal vesicle (GV) is initially decondensed with the nucleolus not surrounded by heterochromatin (the NSN configurations). During oocyte growth, the GV chromatin condenses into perinucleolar rings (the SN configurations) or other corresponding configurations with or without the perinucleolar rings, depending on species. During oocyte maturation, the GV chromatin is synchronized in a less condensed state before germinal vesicle breakdown (GVBD) in species that has been minutely studied. As not all the species show the SN configuration and gene transcription always stops at the late stage of oocyte growth, it is suggested that a thorough condensation of GV chromatin is essential for transcriptional repression. Because the GV chromatin status is highly correlated with oocyte competence, oocytes must end the NSN configuration before they gain the full meiotic competence and they must take on the SN or corresponding configurations to stop gene transcription before they acquire the competence for early embryonic development. In this study, we firstly investigated whether the follicle size could determine chromatin configuration in porcine oocyte. For this experiment, follicles was divided into three groups (<1 mm follicle, 1～3 mm follicle and 3～6 follicle). Using DAPI staining, the GV nucleolus and chromatin of porcine oocytes was classified into SN, SN-NSN and NSN configurations. MⅠ and M Ⅱ of three groups's Mature oocytes by staining was confirmed the configuration of chromatin. The maturation rate and parthenogenetic development potential were significant different between the SN and NSN configurations oocytes. These results indicated that chromatin changes in GV oocytes affect the development potential of porcine embryos.

키워드

Chromatin SN NSN Porcine

저자

  • Min-Gu Lee [ Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pig, Chungnam National University ]
  • Jin-Yu Zhang [ Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pig, Chungnam National University ]
  • Rong-Xun Han [ Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pig, Chungnam National University ]
  • Yun-Fei Diao [ Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pig, Chungnam National University ]
  • Reza K. Oqani [ Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pig, Chungnam National University ]
  • Dong-Il Jin [ Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pig, Chungnam National University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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