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[Poster Presentation] - Cloning

Productions of Transgenic Cloned Minipigs using Freshly Thawed Donor Cells Equilibrated with Roscovitine

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 36 No 2 Supplement (2012.06)바로가기
  • 페이지
    pp.37-37
  • 저자
    Seongsoo Hwang, Keon Bong Oh, Dae-Jin Kwon, Sun A Ock, Jeong-Woong Lee, Jin-Ki Park, Gi-Sun Im
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A178314

원문정보

초록

영어
Here we report the productions of genetically modified cloned Massachusetts General Hospital miniature pig (MGH minipig) using freshly thawed donor cells equilibrated with roscovitine. Fibroblasts were isolated from the ear skin of a 10-day-old male MGH minipig. The donor cells were divided into two groups: cultured for 3 days (culture) and freshly thawed with 500 nM roscovitine. The viability of the donor cells was significantly higher at 0 h (94.6±3.5) compared with 1 h (81.7±5.7) after thawing (p=0.028). After 1 hr of equilibration time, the proportion of G0/G1 stage in roscovitine group was not different from 0 hr group, but not in culture medium group (p<0.01), respectively. Although the developmental characteristics were not different in both methods, the pregnancy and delivery rate in freshly thawed group were significantly higher than that of culture group (p<0.01), respectively. In total, 12 TG cloned MGH minipigs were delivered and the individual cloning efficiency was from 0 to 2.54%. Taken together, the use of freshly thawed donor cells equilibrated with roscovitine may be helpful method to increase the productivity of the genetically modified cloned MGH minipigs.

키워드

Transgenic cloned pigs Cell cycle synchronization Freshly thawed donor cells Roscovitine Cloning efficiency

저자

  • Seongsoo Hwang [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Gyeonggi-do, Republic of Korea ]
  • Keon Bong Oh [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Gyeonggi-do, Republic of Korea ]
  • Dae-Jin Kwon [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Gyeonggi-do, Republic of Korea ]
  • Sun A Ock [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Gyeonggi-do, Republic of Korea ]
  • Jeong-Woong Lee [ Development and Differentiation Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea ]
  • Jin-Ki Park [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Gyeonggi-do, Republic of Korea ]
  • Gi-Sun Im [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Gyeonggi-do, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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