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Characterization of Induced Pluripotent Stem Cells from Wild and Transgenic Minipigs

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 36 No 2 Supplement (2012.06)바로가기
  • 페이지
    pp.25-25
  • 저자
    DaeJin Kwon, Hyelena Jeon, KeonBong Oh, Seongsoo Hwang, SunA Ock, Gi-Sun Im, Jin-Ki Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A178308

원문정보

초록

영어
The generation and application of porcine iPSCs (piPSCs) as a large animal model may enable the test for the efficacy and safety of the therapy in the field of human regenerative medicine. Here, we report the generation of piPSC from wild (a 10-day-old Massachusetts General Hospital miniature pig; MGH minipig) and genetically modified pig, alpha1,3-Galactosyltransferase knock-out (—/—) (GalT KO homo) and human CD46 (membrane cofactor protein) knock-in (hCD46 KI) MGH minipig (a 10-day-old). Fibroblasts were isolated from the ear skin of wild and MGH minipigs, respectively. After 2 passages, each of fibroblasts was transduced with cocktail of 6 human factors (POU5F1, NANOG, SOX2, C-MYC, KLF4, and LIN28) and cultured on a mitotically inactive mouse embryonic fibroblast (MEF) monolayer. Both of reprogrammed somatic cells expressed the classical pluripotency markers (POU5F1, NANOG, and SOX2) and surface marker (SSEA1). Similar to mouse ESCs, both piPSCs from wild and transgenic minipigs were negative for SSEA3, Tra-1-60, and Tra-1-81. Further these cells could form embryoid body (EB) and differentiate into 3 germ layers in vitro (ectoderm: FOXJ3 and PAX6, mesoderm: HAND2, and endoderm: SOX17 and GATA6). Our piPSCs may provide useful source as a large animal model for studying approaches that can reduce an immune- rejection of cell or organ transplantation.

키워드

3-Galactosyltransferase CD46 (membrane cofactor protein) Gene knock in/out Porcine induced pluripotent stem cells (piPSCs) Xenotrasplantation α-1

저자

  • DaeJin Kwon [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Gyeonggi 441-706, Republic of Korea ]
  • Hyelena Jeon [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Gyeonggi 441-706, Republic of Korea ]
  • KeonBong Oh [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Gyeonggi 441-706, Republic of Korea ]
  • Seongsoo Hwang [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Gyeonggi 441-706, Republic of Korea ]
  • SunA Ock [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Gyeonggi 441-706, Republic of Korea ]
  • Gi-Sun Im [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Gyeonggi 441-706, Republic of Korea ]
  • Jin-Ki Park [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Gyeonggi 441-706, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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