Earticle

초록

영어

Acteoside acts as an anti-oxidative activity and anti-apoptosis in the cells. But, it has been not studied on maturation and development of porcine oocytes. The aims of the present study were to examine the effects of acteoside on the morphological progress of meiosis, developmental competence, and ROS in porcine oocytes. Oocytes were matured in tissue culture medium-199, supplemented with acteoside at various concentrations: 0 (control), 10, 30 and 50 μM. The oocytes maturation rates of groups supplemented with acteoside were no significantly different (81.13, 85.96, 82.95 and 83.68%, respectively). Level of ROS was significantly decreased in acteoside treated group. Furthermore, the parthenogenetic blastocyst rate was significantly improved in 10 μM acteoside treated group compared with control group (44.83 vs. 27.75%). And we investigated effect of acteoside on the oocytes condition represented by cytoplasmic maturation by homogeneous distribution and formation of cytoplasmic organelles and regulation of apoptosis-related genes. In the results. during IVM, 10 μM acteoside treated oocytes showed that the mitochondria and lipid droplet were smaller and homogeneous distribution in cytoplasm compare with control oocytes. And reverse transcription polymerase chain reaction (RTPCR) of parthenogenetic blstocysts revealed that acteoside increased the anti-apoptotic genes (Mcl-1, Bcl-2 and Bcl-xL), whereas reduced the expression of pro-apoptotic genes (Bax and Bak). In conclusion, based on the results, the effect of acteoside on IVM was not attractive. However, in acteoside treated group, cytoplasmic maturation seemed to be improved with morphologically uniform distribution of cytoplasmic organelles. Furthermore, embryonic development in acteoside treated group was significantly highly increased than that of non-treated group. Our results represents that addition of acteoside to the IVM medium has a beneficial effect in physiology of porcine oocytes, providing a improved method for porcine oocytes in vitro. * This work was supported by a grant (Code# PJ008148) from BioGreen21 Program, Rural Development Administration, Republic of Korea.

키워드

저자

• Keun Jung Kim [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
• Ji Hye Lee [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
• Xiaoxia Li [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
• Eun Young Kim [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
• Youn Bae Park [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
• Kang Sun Park [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
• Yu-Na Ha [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
• Maria Kim [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
• Kil Woo Han [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
• Jun Jong Baek [ Chungnam livestock Research Institute, Cheongyang-Gun, Chungcheongnam-do, Korea ]
• Jae Sam Lim [ Chungnam livestock Research Institute, Cheongyang-Gun, Chungcheongnam-do, Korea ]
• Jae Seok Woo [ National Institute of Animal Science, Suwon, Gyeonggi-do, 441-706, Korea ]
• Min Kyu Kim [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & Developmental Biology(Supplement)

• 간기

  연간

• 수록기간

  2001~2017

• 십진분류

  KDC 527 DDC 636