Earticle

초록

영어

The objective of the current study was to describe in vitro embryo production in Hanwoo, analyzing oocytes yield and embryo production. The effects of oocytes production and the number of OPU procedures per animal on embryo production were also evaluated. OPU was done every 3～4 days during experimental period and collected oocytes were fertilized in vitro in both OPU and needle puncture groups. First, we compared the recovery rate of oocytes based on OPU session (Experiment 1). The average of collected oocytes was calculated from every 10 session. The average number of total oocytes recovered per animalonsessionwas 5.16 (mean). Second, we compared the recovery rate base on collection period of OPU (Experiment 2). The following results show the difference of the number of recovered oocytes in every month during the procedure between the months of session. Every animal shows the constant number of recovered oocytes for the first 5 months. However, the recovery rate of oocytes was decreased from month 6 to 8. Third, we compared the developmental rate to blastocyst in two groups (Experiment 3). Oocytes by needle puncture were fertilized with frozen-thawing semen; the cleavage rate 24～48 h after in vitro fertilization (IVF) was 75.8% and blastocyst development rate was 18.8% in needle puncture group. Even though there is lower cleavage rate after IVF in OPU group (61.1%), blastocyst development rate was higher compared with needle puncture group (28.4%). In conclusion, Blastocyst developmental rate could be increased by OPU than classical method of needle puncture. Improvement of bio- technique in collecting oocytes could be applied to understand the reproductive physiology in cattle, expecially Hanwoo. Therefore, further investigation should be done to clarify the efficiency and advantage of OPU involved in reproduction in animals and human being.

키워드

저자

• Youn Bae Park [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
• Keun Jung Kim [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
• Ji Hye Lee [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
• Kang Sun Park [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
• Eun Young Kim [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
• Maria Kim [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
• Kil woo Han [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
• Xiaoxia Li [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
• Jeong Yu [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
• Dong Soo Lee [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
• Min Kyu Kim [ Department of animal science & biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & Developmental Biology(Supplement)

• 간기

  연간

• 수록기간

  2001~2017

• 십진분류

  KDC 527 DDC 636