Earticle

초록

영어

To determine the biological effects of low‐dose‐rate radiation (137Cs, 2.95 mGy/h) on EL4 lymphoma cells during 24 h, we investigated the expression of genes related to apoptosis, cell cycle arrest, DNA repair, iron transport, and ribonucleotide reductase. EL4 cells were continuously exposed to low‐dose‐rate radiation (total dose: 70.8 mGy) for 24 h. We analyzed cell proliferation and apoptosis by trypan blue exclusion and flow cytometry, gene expression by real‐time PCR, and protein levels with the apoptosis ELISA kit. Apoptosis increased in the low‐dose‐rate irradiated cells, but cell number did not differ between non‐ (Non‐IR) and low‐dose‐rate irradiated (LDR‐IR) cells. In concordance with apoptotic rate, the transcriptional activity of ATM, p53, p21, and Parp was upregulated in the LDR‐IR cells. Similarly, Phospho‐p53 (Ser15), cleaved caspase 3 (Asp175), and cleaved Parp (Asp214) expression was upregulated in the LDR‐IR cells. No difference was observed in the mRNA expression of DNA repair‐related genes (Msh2, Msh3, Wrn, Lig4, Neil3, ERCC8, and ERCC6) between Non‐IR and LDR‐IR cells. Interestingly, the mRNA of Trfc was upregulated in the LDR‐IR cells. Therefore, we suggest that short‐term low‐dose‐rate radiation activates apoptosis in EL4 lymphoma cells.

목차

ABSTRACT
 1. INTRODUCTION
 2. MATERIALS AND METHODS
 3. RESULTS AND DISCUSSION
 4. CONCLUSION
 REFERENCES

키워드

저자

• JIN JONG BONG [ Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd, ]
• YU MI KANG [ Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd, ]
• SUK CHUL SHIN [ Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd, ]
• MOO HYUN CHOI [ Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd, ]
• SEUNG JIN CHOI [ Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd, ]
• KYUNG MI LEE [ †Global Research Lab, BAERI Institute, Department of Biochemistry and Molecular Biology, Korea University College of Medicine ]
• HEE SUN KIM [ Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd, Korea University College of Medicine, ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  방사선방어학회지 [Journal of Radiation Protection and Research]

• 간기

  계간

• pISSN

  2508-1888

• 수록기간

  1976~2026

• 등재여부

  KCI 등재,SCOPUS

• 십진분류

  KDC 559 DDC 629