In this study, we evaluated the effects of the crude extract purities and pure paclitaxel contents on the efficiency and behavior (purity, yield, fractional precipitation time, precipitate shape and size) of fractional precipitation for the purification of paclitaxel from plant cell cultures. As the purity of crude extract and pure paclitaxel content were increased, the yield and purity of paclitaxel were increased and the precipitation time and precipitate size were decreased. Furthermore, we found that the maximum amount of pure paclitaxel content for fractional precipitation was 0.45~0.51% (w/v) regardless of purity of crude extract. By increasing the surface area per working volume (S/V) using a variety of ion exchange resins, fractional precipitation time could be reduced significantly as well as improvement of yield and purity of paclitaxel. Use of an ion exchange resin also resulted in the production of smaller paclitaxel precipitates since it inhibited the growth of particles.
키워드
PaclitaxelFractional precipitationCrude extract purityPure paclitaxel contentSurface area per working volume (S/V)
저자
Ji-Yeon LEE [ Department of Chemical Engineering, Kongju National University, Cheonan, 330-717. ]
Jin-Hyun KIM [ Department of Chemical Engineering, Kongju National University, Cheonan, 330-717. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동