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Streptomyces sp. strain BCNU 1001 was isolated from forest soil samples. Cultural, morphological, and physiological characteristics as well as 16S rDNA analysis revealed that the isolate, BCNU 1001, belonged to the genus Streptomyces. The antimicrobial activity of the ethyl acetate extract was confirmed using the broth microdilution technique. The minimum inhibitory concentration (MIC) of the BCNU 1001 ethyl acetate extract was 0.25 mg/ml for Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa, and 0.125 mg/ml for Micrococcus luteus, Staphylococcus aureus, and Pseudomonas fluorescens. The MIC of the BCNU 1001 ethyl acetate extract for Aspergillus niger, Candida albicans, and Saccharomyces cerevisiae was 0.5 mg/ml, 0.125 mg/ml, and 0.25 mg/ml, respectively. BCNU 1001 was also active against dermatophytic fungi such as Trichophyton mentagrophytes and T. rubrum. Furthermore, BCNU 1001 was also found to be effective against Methicillin-resistant Staphylococcus aureus (MRSA), and its ethyl acetate extract showed MIC = 0.5 mg/ml against MRSA. The most abundant antimicrobial compound was identified as a 2-hydroxybenzyl alcohol through analysis utilizing a nuclear magnetic resonance spectroscopy. This compound was seen to be very effective against some kinds of bacteria and fungi.( This research was financially supported by the Korean Ministry of Education, Science Technology (MEST), and Korea Institute for Advancement of Technology (KIAT) through the Human Resource Training Project for Regional Innovation)

키워드

Antibacterial activity Antifungal activity methicillin-resistant Staphylococcus aureus Streptomyces sp. BCNU 1001Streptomyces sp. strain BCNU 1001 was isolated from forest soil samples. Cultural morphological and physiological characteristics as well as 16S rDNA analysis revealed that theisolate BCNU 1001 belonged to the genus Streptomyces. The antimicrobial activity of the ethylacetate extract was confirmed using the broth microdilution technique. The minimum inhibitoryconcentration (MIC) of the BCNU 1001 ethyl acetate extract was 0.25 mg/ml for Bacillus subtilis Escherichia coli and Pseudomonas aeruginosa and 0.125 mg/ml for Micrococcus luteus Staphylococcus aureus and Pseudomonas fluorescens. The MIC of the BCNU 1001 ethyl acetateextract for Aspergillus niger Candida albicans and Saccharomyces cerevisiae was 0.5 mg/ml 0.125 mg/ml and 0.25 mg/ml respectively. BCNU 1001 was also active against dermatophyticfungi such as Trichophyton mentagrophytes and T. rubrum. Furthermore BCNU 1001 was alsofound to be effective against Methicillin-resistant Staphylococcus aureus (MRSA) and its ethylacetate extract showed MIC = 0.5 mg/ml against MRSA. The most abundant antimicrobialcompound was identified as a 2-hydroxybenzyl alcohol through analysis utilizing a nuclearmagnetic resonance spectroscopy. This compound was seen to be very effective against somekinds of bacteria and fungi.( This research was financially supported by the Korean Ministry ofEducation Science Technology (MEST) and Korea Institute for Advancement of Technology(KIAT) through the Human Resource Training Project for Regional Innovation) 2-hydroxybenzyl alcohol

저자

• Hye Jung CHOI [ Interdisplinary Program for Biotechnology and Department of Biology, Changwon National University, Changwon 641-773, Korea. ]
• Young Whan CHOI [ Department of Horticultural Bioscience, Pusan National University, Miryang 627-706, Korea. ]
• Yong Kee JEONG [ Department of Biotechnology, Dong-A University, Busan 604-714, Korea. ]
• Woo Hong JOO [ Interdisplinary Program for Biotechnology and Department of Biology, Changwon National University, Changwon 641-773, Korea. ]

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