Fullerene is a form of carbon molecule that has wide application for the various fields of industry. Stable aqueous dispersions of C60 fullerene called nano-C60. We synthesized nano-C60 aggregates with different size ranges to determine their cytotoxicity in human cell lines derived from liver, lung, and skin. The nano-C60of size was controlled in the range of about 80 to 300 nm diameter.We confirmed that the differences in size distribution of C60 colloidal suspension are maintained in the presence of 10% fetal bovine serum for up to 48 hours and that their cytotoxicity is size-dependent. The cytotoxicity of nano-C60 is known that caused by reactive oxygen species (ROS) generation. Nano-C60-induced ROS reached the maximum concentrations at 6 hours in phosphate-buffered saline (PBS). The amount of ROS in PBS and the oxidative stress induced by C60exposure are also size-dependent.
키워드
FullereneC60nano-C60ROS
저자
Yoshie ARAI [ Dept. of Life Science, Dongguk University, Seoul, Korea 100-715. ]
Eun-Ha SHIM [ Dept. of Chemistry, Dongguk University, Seoul, Korea 100-715. ]
Hyun JUNG [ Dept. of Chemistry, Dongguk University, Seoul, Korea 100-715. ]
Wonhee JANG [ Dept. of Life Science, Dongguk University, Seoul, Korea 100-715. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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