A method is described for the characterization of sexual transmitted diseases (STD) infections by restriction fragment mass fingerprint analysis. Through analysis of over 35,000 clinical cases, 14 pathogens which are most abundantly found among Koreans were selected and primers containing restriction enzyme region and mass fragment sequences according to each type of pathogen were designed by considering their sequences. A multiplex PCR method was established for the diagnosis of multiple infections to STD. Amplified genomic nucleic acids encoding the pathogenicity-determining region of all pathogens were digested with a restriction enzyme, and the restriction fragments were analyzed by matrix-assisted laser desorption-ionizationtime- of-flight mass spectrometry (MALDI-TOF MS). This method employed to diagnose reliably both single and multiple infections in clinical human samples for 14 target pathogens. The procedure described is rapid, inexpensive and compatible with automation.
키워드
STDMALDI-TOFrestriction enzyme
저자
Tae Won KIM [ Dept. of Chemical and Biomolecular Engineering, KAIST, Daejeon. ]
Yun Kyung JUNG [ Dept. of Chemical and Biomolecular Engineering, KAIST, Daejeon. ]
Hyun Gyu PARK [ Dept. of Chemical and Biomolecular Engineering, KAIST, Daejeon. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
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