Earticle

초록

영어

We described the fabrication method of microfluidic channels with circular-cross sectional geometries by reflow phenomenon of thick positive photoresist. Rectangular shaped positive photoresist micropatterns on a Si wafer were fabricated by a photolithography and then melted by heating at 105 °C, which leads to shape change from rectangular to semicircular. For the perfect cylindrical micropatterns, we optimized the reflow conditions by using positive photoresist and the resultant circular micropatterns were used as a template for generating a PDMS circular microchannels. The diameter can be varied from several to several hundred micrometers, and the channel routes can be easily patterend such as straight, S-curve, x-shape, cross shapes, Y and T-shape for mimicking the for vascular network.To form the artificial vascular network, we cultured the HUVECs inside the PDMS micro circular channels to make the endothelial layer. Similar to the in-vivo situations, HUVEC cells were adhered, proliferated and aligned to the direction of the microchannel, suggesting that the simple reflow method can produce a PDMS circular microchannels to mimick the in-vivo like 3D artificial blood vessel.

키워드

저자

• Jong Seob CHOI [ Dept. of Chemical and Biomolecular Engineering, KAIST, Daejeon, 305-701. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576