Earticle

초록

영어

Contractile force of myotubes is one of key parameters for studying diseases related to muscles. However, there is lack of an efficient and affordable device for generating single myotubes and quantifying the contractile force of each single myotube in vitro. In this study, we describe a novel cell culture device equipped with elastic posts for measuring contractile force generated by a single myotube in situ. The microchannel cell culture system is composed of an array of microchannels with a dimension of 50 μm (width) x 500 μm (length) x 100 μm (height) having PDMS (polydimethyl siloxane) pillars with a dimension of 30 μm (diameter) x 100 μm (height) located at both ends of each channel. When C2C12 myoblast cells were cultured in the microchannels for several days, they were fused to form multi-nucleated single myotubes and then started to be attached to pillars in the channel. Since the contractile force of each myotube can be calculated from the bending degree of micropillars pulled by a single myotube, it is expected that our device can be useful tool for studying the mechanical characteristics of myotubes in vitro.

키워드

저자

• Yinhua LI [ Dept. of Chemistry and Nanoscience, Ewha Womans University, Seoul, 120750. ]
• Keiko KAWAUCHI [ Mechanobiology Institute, National University of Singapore, Singapore. ]
• Benoit LADOUX [ Mechanobiology Institute, National University of Singapore, Singapore 2. Université Paris Diderot &CNRS, Paris, France. ]
• Hideaki FUJITA [ Riken Quantitative Biology Center, Osaka, Japan. ]
• Sungsu PARK [ Department of Chemistry and Nano Science, Ewha Womans University, Seoul, Korea 2. Mechanobiology Institute, National University of Singapore, Singapore. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576