In this study, cell chip was fabricated with conductive composite film composed of RGD peptide and carbon nanotubes (CNTs) for improving conductivity of RGD peptide film on gold electrode. The topological characteristics of modified electrode surface were validated by atomic force microscopy (AFM) and scanning electron microscopy (SEM). Human embryo kidney (HEK293T) cells were immobilized on fabricated cell chip to detect electrochemical characteristics by cyclic voltammetry. The electrochemical sensitivity of fabricated electrode evidently increase compared with bare gold and RGD film coated electrode. Thereafter, 2 kinds of nephrotoxic antibiotics were treated to HEK293T cells which were immobilized on modified electrode in cell chip. The electrochemical signal intensities linearly decreased with increase of antibiotics’ concentration. These results were confirmed by MTT assay. Our newly developed renal cell chip can be used in drug development field as label-free screening device to detect nephrotoxic effects of antibiotics on kidney. Acknowledgement: This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (2012-0000163) and by the Nano/Bio Science &Technology Program (M10536090001-05N3609- 00110) of the Ministry of Education, Science, and Technology (MEST), and by Korea Institute for Advancement in Technology (KIAT) through the Workforce Development Program in Strategic Technology.
키워드
Cell ChipRGD peptideCarbon nanotubes
저자
Hyeon-Yeol CHO [ Department of chemical &Biomolecular Engineering, Sogang University, Seoul 121-742, Korea. ]
Tae-Hyung KIM [ Department of chemical &Biomolecular Engineering, Sogang University, Seoul 121-742, Korea. ]
Jeong-Woo CHOI [ Department of chemical & Biomolecular Engineering and Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul 121-742, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동