Magnetic beads modified with DNA intercalating agents have been developed for simple separation of DNA in solution after PCR by Microwave reactor. It is widely reported that a microwave system could enhance the chemical and biological reactions. An uniform type of microwave system for biological and mild chemical reaction has been developed and applied for a synthetic and some enzymatic reactions. In this study, We modified the bare magnetic particle to 9-aminoacridine coated magnetic particle using conventional method and microwave assisted method. As a result, we confirm, when we reacted with microwave systems, that 9-aminoacridine coated magnetic was increased reaction efficiency in short time(<30min). We developed and confirmed the microwave system that increases the reaction efficiency of surface modification on magnetic particles and shortens the time for reaction.
키워드
DNA intercalating agentMicrowave ovenDNA separationMagnetic bead
저자
Hyoyoung MUN [ Dept. of photoincs & applied Physics, GIST, Gwangju, 550-712. ]
Mingon KIM [ Dept. of photoincs & applied Physics, GIST, Gwangju, 550-712. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동