Earticle

초록

영어

In the past decade, many analysis methods of DNA mismatch detection have been proposed for analyzing SNPs (single nucleotide polymorphisms), which are one-base DNA sequence variations that are responsible for various inherited diseases and drug resistance. Nevertheless, ligase-based assay is more proper methods in terms of various applications then other SNP analysis methods. However, DNA ligase-based methods for mismatch detection showed unavoidable limitation in cytosine/thymine (C/T)-type SNP analyses. Theoretically, ligation product should be detected for guanine:cytosine (G:C) match and not for guanine:thymine (G:T) mismatch. But, C/T type SNP analysis is not distinguished clearly due to false-positive induced by hydrogen bondings between G:T. In this study, DNA oligomers containing modified nucleobases (5-Nitroindole, dSpacer, Iso-dG, and Iso-dC) at the end were employed as new ligation fragments for DNA-ligase-based SNP analysis. When such modified-end DNA ligation fragments are used, false-positive result for G:T mismatch was not found both using T4 DNA ligase and Thermostable DNA ligase (Tth DNA ligase). This ligation method using modified-end DNA ligation fragment has potential for the accurate analysis of C/T-type SNPs.

키워드

저자

• Eui Kyoung JANG [ Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam 339-700, Korea. ]
• Ki Baek YEO [ Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam 339-700, Korea. ]
• Mi Ran KI [ Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam 339-700, Korea. ]
• Ki Ha MIN [ Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam 339-700, Korea. ]
• Seung Pil PACK [ Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam 339-700, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576