Earticle

초록

영어

Low protein yield has been known as a main obstacle in transgenic plant cell cultures for the production of recombinant protein. Production of a fusion protein, human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig), was induced by sugar starvation using rice alpha-amylase 3D (RAmy3D) promoter in transgenic rice cell suspension cultures. Due to the sugar depletion, RAmy3D promoter causes cell death during the production phase. Under this condition, the rice cells can be more sensitive to hydrodynamic stress. In flask cultures, hydrodynamic stress can be changed by the parameters such as the size of flasks, agitation rate and working volume. In this study, hCTLA4Ig productivity was compared between the cultures in different flask sizes (0.1, 0.25, 0.5, 1, 2 and 5 L). In larger flasks, significant decrease of hCTLA4Ig level was observed and this decrease was thought to be originated from the changes in hydrodynamic stress. To confirm the effect of hydrodynamic stress on hCTLA4Ig production in flask cultivation, both agitation rate and working volume were changed in 1-L flask. It was confirmed that hydrodynamic stress could lower the production of hCTLA4Ig as well as cell viability. In conclusion, hydrodynamic stress should be controlled to improve the hCTLA4Ig production during the scale-up process.

키워드

저자

• Jun Yong PARK [ Department of Biological Engineering, Inha University, Incheon 402-751. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576