Gi-Hong JIN, Jun-Young KWON, Hyung-Jin NAM, Myoung-Sik KIM, Jun-Yong PARK, Sun-Hee JEONG, Yun-Young PAK, Min-Sub KIM, Hong-Youl CHOI, Dong-Il KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A174804
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원문정보
초록
영어
Plant-derived peptones have been utilized to improve cell growth and product formation in plant cell cultures over 10 years. Supplementation of defined medium with plant-derived peptone was found to enhance cell growth and the production of human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) significantly in transgenic rice cell suspension cultures. Production of hCTLA4Ig was induced by sugar starvation. Nitrogen (N) and carbone (C) sources have been known as major factors in plant cell cultures. Therefore, it was necessary to investigate the effects of C and N sources for the improvement of protein production. In addition, optimization of the concentrations of C and N sources is required. As a carbon source, 0 (control), 3, 5% (w/v) of sucrose was added in 100-mL Erlenmeyer flasks with 1% plant-based peptone. In case of adding 5% sucrose, the maximum dry cell weight and hCTLA4Ig production were higher than those of control culture. In conclusion, it was found that the determination of optimal ratio between C and N sources are important for the production of recombinant protein in transgenic rice cultures.
키워드
hCTLA4IgCell CulturesPlant-derived Peptone
저자
Gi-Hong JIN [ Department of Biological Engineering, Inha University253 Yonghyundong, Nam-gu, Incheon, Korea. ]
Jun-Young KWON [ Department of Biological Engineering, Inha University253 Yonghyundong, Nam-gu, Incheon, Korea. ]
Hyung-Jin NAM [ Department of Biological Engineering, Inha University253 Yonghyundong, Nam-gu, Incheon, Korea. ]
Myoung-Sik KIM [ Department of Biological Engineering, Inha University253 Yonghyundong, Nam-gu, Incheon, Korea. ]
Jun-Yong PARK [ Department of Biological Engineering, Inha University253 Yonghyundong, Nam-gu, Incheon, Korea. ]
Sun-Hee JEONG [ Department of Biological Engineering, Inha University253 Yonghyundong, Nam-gu, Incheon, Korea. ]
Yun-Young PAK [ Department of Biological Engineering, Inha University253 Yonghyundong, Nam-gu, Incheon, Korea. ]
Min-Sub KIM [ Department of Biological Engineering, Inha University253 Yonghyundong, Nam-gu, Incheon, Korea. ]
Hong-Youl CHOI [ Department of Biological Engineering, Inha University253 Yonghyundong, Nam-gu, Incheon, Korea. ]
Dong-Il KIM [ Department of Biological Engineering, Inha University253 Yonghyundong, Nam-gu, Incheon, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동