Earticle

초록

영어

Three-dimensional (3D) cell culture systems have been fabricated using a magnetic force [1], scaffolds [2], and biocompatible gels [3]. In this report, fibroin microcapsules approximately 500 nm in diameter were fabricated to entrap iron oxide nanoparticles (Fe3O4) and introduced to 3T3 cells cultured in vitro. Magnetized cells were levitated using a neodymium magnet placed on the top of the culture plate containing trypsin-treated 3T3 cell (6.2X105 cells) and further incubated for three days with daily media change. Disk-shaped dendroid cell clumps were formed up to 2400 mm2, which were analyzed using confocal laser scanning microscope (CLSM) and flow cytometry to evaluate cell numbers, shape and viability. The CLSM im ages of fluorescence stained cells indicate that the 3D cell clumps were concave and full of cells. Flow cytometry shows that the morphology of cells in the 3D cell clumps was significantly influenced. Viability of the cell clumps decreased with the increasing culture period but was not influenced by the increasing amount of microcapsules. The results indicate that mass transfer limitation is critical to maintain viability of 3D cell culture.

키워드

저자

• Joon-Ho LEE [ Dept. of Bioengineering and Technology, Kangwon Natonal University, Chunchon, 200-701. ]
• Won HUR [ Dept. of Bioengineering and Technology, Kangwon Natonal University, Chunchon, 200-701. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576