Earticle

초록

영어

Phosphodiesterases (PDEs) are involved in many cellular signal transductions mediated by cAMP. In addition, PDEs are involved in phosphorylation when the precursors of soluble lysosomal enzymes transport into the lysosomes. To certify the relation between lysosomal activity and PDEs, Bovine Aortic Endothelial cells (BAECs) transfected with 200, 300 and 400 pmol antisense of PDE4A, B, C and D. Through cytochemical analysis of lysosomes with Lyso-Tracker, we demonstrated that the number of and fluorescent intensity of lysosomes in BAECs, and the endothelial nitric oxide synthase (eNOS), PDE4A, B, C, D and cathepsin B were quantitatively analyzed by western blotting. We found that the number of and fluorescent intensity of lysosomes decreased only the case of PDE4C knock-out, but was not changed by other PDE series. In addition, protein expression of PDE4A, B, C, and D were decreased by transfection but eNOS and cathepsin B were reduced by transfection of PDE4C antisense. Therefore, our results suggest that only PDE4C would affect the activity of lysosomal enzymes in cells.

키워드

저자

• Jihee YOON [ 1Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14 Deokjin-dong, 1Ga Deokjin-Gu Jeonju 561-756, South Korea. ]
• Hyun-Ju UM [ Department of Microbiology, Chungbuk National University, 410 Sungbong-Ro, Heungduk-Gu, Cheongju 361-763, South Korea. ]
• Sang-Hee LEE [ Department of Microbiology, Chungbuk National University, 410 Sungbong-Ro, Heungduk-Gu, Cheongju 361-763, South Korea. ]
• Jiho MIN [ 1Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14 Deokjin-dong, 1Ga Deokjin-Gu Jeonju 561-756, South Korea. ]

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  한국생물공학회 학술대회

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  1985~2013

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