Earticle

초록

영어

In this study we tried to make a silk scaffold contained nano-HAp for dental tissue engineering. The silk scaffolds extracted sericin were coated with 0.3g, 0.15g, 0.03g of nano-HAp dissolved in PBS. Then, they were soaked in a 1% type I atelocollagen solution and air dried. They were crosslinked with 0.02% carbodiimide and lyophilized for 48 h. And they were sterilized by γ- irradiation at 10 KGy. DPSCs were seeded into silk scaffolds contained nano-HAp at a density of 2.8x104 cells/cm2 and cultured for 3weeks in growth medium. Then, they were cultured for 4weeks in differentiation medium and were transplanted in the nude mouse. The biopsy was processed at 8weeks. Col III and fibronectin, osteocalcin, osteopontin, osteonectin, osteoprotegerin and BMP-2 levels in the culture were greatest in the 0.15g of HAp. The calcification and the revelation of ostecalcin and osteopontin were better made in the silk scaffold contained 15mg of nano-HAp. We could make silk scaffold contained various concentration of nano-HAp. Among them, 15mg of nano-HAp was the most effective for osteogenesis. It will be a suitable substrate as biomaterial for bone tissue .

키워드

저자

• Mi-Jung HAN [ Dongguk University Research Institute of Biotechnology. ]
• Young-Kwon SEO [ Dongguk University Research Institute of Biotechnology. ]
• Hee-Hoon YOON [ Dongguk University Research Institute of Biotechnology. ]
• Kye-Yong SONG [ Department of Pathology, Chung-Ang University. ]
• Jung-Keug PARK [ Department of Medical Biotechnology, Dongguk University. ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576