Kieun CHOI, Byoung Seung JEON, Han min WOO, Byoung-In SANG
언어
영어(ENG)
URL
https://www.earticle.net/Article/A174780
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원문정보
초록
영어
Megasphaera elsdenii NCIMB 702410 is a gram-negative strictly anaerobic bacterium isolated in the rumen of cattle and sheep. This strain is able to utilize sucrose to produce 4.53 g/L of hexanoic acid in a batch culture. However, high titer of hexanoic acid is toxic to the cellular growth. Therefore, the objective of this study is to improve hexanoic acid production of Megasphaera elsdenii by using reactive extraction method. The extraction of hexanoic acid was performed with 10 % (v/v) Alamine 336 in Oleyl alcohol as a solvent and this method led the selective removal of hexanoic acid from the fermentation broth with high recovery rate. Compared to conventional fermentation, in situ extractive fermentation showed a 5.7-folds higher production of hexanoic acid (25.83 g/L) due to immediate extraction of hexanoic acid. In addition, this extraction method was applied to fed-batch fermentation, resulting that the titer of hexanoic acid was increased up to about 70.04 g/L (15.5-folds higher). In conclusion, the efficient extraction process is crucial for fermentative production of hexanoic acid by Megasphaera elsdenii NCIMB 702410 using sucrose as a sole carbon.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동